The growth of Neisseria gonorrhoeae on brain heart infusion blood agar in which the base was sterilized by filtration has been compared with growth on the same medium sterilized by heat. Colonies were larger on the unheated medium, and autoclaving at 115 degrees C of 121 degrees C for 15 minutes was accompanied by a progressive decrease in colony size. Viable counts on the three media showed no...

in all biological systems growth is defined as increase of chemical compounds. bacteria can achieve to balanced growth if they are growing in a medium, which are completely adapted to it. clostridium chauvoei, (clostridium feseri) is an anaerobic, spore forming, motile, and polymorph bacteria, which its size varies from 0.5-1 to 3-8 micron and could be observed as individual bacterium, diplo, a...

This study was conducted to investigate the effect of tofu wastewater (TW) addition to the growth medium on the growth of Spirulina platensis. The TW addition was varied in range of 0 – 8 %v/v. The results showed that the growth rate (μ) of S. platensis at TW addition of 0, 2, 4, 6, 8 %v/v was 0.007, 0.084, 0.074, 0.088, 0.086 mg/L/d, respectively. The medium with TW content of 6 %v/v (carbon:n...

  In 1926 for the first time Dobell and laid low synthesized and used Hsre+ s ( horse serum, ringer, egg+ starch rice) as a culture medium for growth of E.histolytica. since then this medium along with tens of other media have been used for growth of E.histolytica and other intestinal microorganisms. This medium has been used worldwide in many diagnostic and research laboratories. In Iran, in ...

BACKGROUND Laboratory diagnosis of tuberculosis has undergone a rapid change during last few years and a number of techniques for culture as well as molecular diagnosis have been used with their respective advantages and disadvantages. Sporadic studies have also reported the isolation of M. tuberculosis on standard blood agar (BA), which at one time was not considered as a suitable medium for m...

sugar type and growth regulators are essential fundamental components of tissue culture media. in this study, different concentrations of some carbohydrate and growth regulators during anther culture and producing somatic embryo of datura stramonium l. was investigated in ms medium. the ms medium supplemented with 0.5 mgl-1 kinetin and 2 mgl-1 2,4-d, and also the medium supplemented with 0.25 m...

OBJECTIVE Urine is the most frequent specimen received for culture/sensitivity by clinical laboratories. The microbiological performance of HiCrome UTI agar medium was compared with Blood agar and MacConkey agar for isolation and presumptive identification of bacteria from urine culture. METHODS A total of 443 consecutively collected midstream and/or catheter-catch urine samples from patients...

background and objectives: availability and uptake of mineral by explants are recognized as being important in tissue culture. the limiting factor for maximum mineral uptake and optimum growth is that minerals are not enough available to the explants. there are the inter-relationships between plant growth, mineral uptake, medium water potential, and mineral movement through the medium. plantlet...

Background: The aim of the present study was to develop a three-dimensional (3D) collagen gel culture system for the in vitro growth and survival of buffalo preantral follicles with or without growth factors. Materials and Methods: Buffalo ovaries were collected from a local abattoir and preantral follicles were isolated through microdissection. Isolated preantral follicles were put either in c...

results however the maximum production of avermectin b1b (17 mg/l) was obtained by using sm2 growth medium containing soluble corn starch, yeast extract, kcl, caco3 and mgso4 which was detected qualitatively by using tlc and quantitatively by hplc. conclusions maximum production was observed with initial medium ph of 7, 10% inoculum size with incubation temperature of 31°c for 10 days of fermen...