“Click” dendrimer-Pd NPs as peroxidase enzyme mimics of H2O2 sensing using o-phenylenediamine oxidation by to 2,3-diaminophenazine.

At suboptimal H202 concentrations, DPNH inhibits the peroxidase activity of the flavoprotein, DPNH peroxidase, by converting the enzyme to an unstable intermediate that decays slowly to inactive enzyme. It is postulated that at concentrations of DPNH that are saturating for peroxidase activity, this unstable intermediate is responsible for most of the DPNH oxidation that is supported by alterna...

Spent mushroom substrate (SMS) from Pleurotus ostreatus is residue of cultivation that known to have lignocellulolytic enzyme activity in it. Enzyme activities provides information on evidence fungi converting complex substrates simple ones as nutrients for them. In this study, the was found cellulase, lignin peroxidase, and protease. The samples used consisted three SMS were collected Padang P...

Wild-type recombinant horseradish peroxidase purified and refolded from Escherichia coli inclusion bodies has been studied in the system of bis(2-ethylhexyl)sulphosuccinate sodium salt (Aerosol OT)-reversed micelles in octane. In contrast with native horseradish peroxidase the wild-type recombinant enzyme forms dimeric structures as judged by sedimentation analysis. Peroxidase substrates affect...

Carambola is a fruit grown in tropical and subtropical regions of the world. Natural antioxidants including vitamin C, carotenoids, certain phenolic substances are abundant carambola fruit. As support health by acting as nutraceutical functional food additives, they help preserve preventing oxidation processes. The various organic or inorganic substrates hydrogen peroxide peroxides terminal oxi...

This study was conducted inside the wire house of Department Biology/ College Education for Pure Science/ Mosul University agricultural season 2018- 2019 to test effect vegetative and root residues Medicago satival on chemical content antioxidant enzyme fenugreek plant Trigonella foenum- graecum L. (Iraq Indian) growth under three different levels field capacity (25, 50 75%). The experiment des...

1. Physical studies of complex-formation between cytochrome c and yeast peroxidase are consistent with kinetic predictions that these complexes participate in the catalytic activity of yeast peroxidase towards ferrocytochrome c. Enzyme-ferricytochrome c complexes have been detected both by the analytical ultracentrifuge and by column chromatography, whereas an enzyme-ferrocytochrome c complex w...

in this study, we investigated the cardioprotective effects of resveratrol. rats were intraperitoneally administered with resveratrol (25 mg/kg bw) or vehicle (ethanol 10%) for 7 days and their heart subjected to ischemia/reperfusion injury. isolated hearts were langendorff perfused, left ventricular functions as heart rate and developed pressure, as well as, heart antioxidant status were deter...

in the present study, some techniques were used for soybean peroxidase (sbp) purification including: ammonium sulfate fractionation, deae sephadex anion exchange chromatography and concanavalin a sepharose 4b affinity chromatography. molecular weight of purified sbp was estimated about 44 kda by sds-page as a single polypeptide band. the optimal ph and temperature for enzyme activity were found...

The goal of the study was to monitor the antioxidative effect of stobadine derivative in the conditions of ischemia-reperfusion of laboratory rat kidney tissue. The animals were divided by random selection into 5 groups (n = 10). The treated groups were given stobadine derivate in peroral doses of 5, 10 and 20 mg/kg in 0.5 % solution of Avicel once a day; the placebo group was given only the so...