Globodera spp. eggs go through a diapause, which remains dormant until favorable hatching conditions are reached. Because of the regulatory concerns with cyst nematodes, it is often only possible to rear eggs for research in the greenhouse. However, hatch is often lower for greenhouse-produced eggs than for eggs obtained from the field. The goal of this research was to determine storage conditi...

A survey was performed to detect the presence of cyst nematodes in the Cape Floristic Region of South Africa. Soil was collected in the rhizosphere of the dominant plant species within blocks of indigenous vegetation and cysts were extracted from them. A total of 81 blocks of indigenous vegetation were sampled as described. Cysts were detected in 7 of these samples, representing 6 different veg...

We sequenced the entire coding region of the mitochondrial genome of Heterodera glycines. The sequence obtained comprised 14.9 kb, with PCR evidence indicating that the entire genome comprised a single, circular molecule of approximately 21-22 kb. The genome is the most T-rich nematode mitochondrial genome reported to date, with T representing over half of all nucleotides on the coding strand. ...

Field microplot experiments were conducted from 1995 to 1998 to determine the relationship between fresh shoot weight of stalk-cut broadleaf and shade-grown cigar wrapper tobacco types (Nicotiana tabacum L.) and initial density of Globodera tabacum tabacum second stage juveniles (J2) per cm(3) soil. Total shoot weight was negatively correlated with initial nematode densities of 12.3 to 747.3 J2...

Potato cyst nematodes (PCNs), including Globodera rostochiensis (Woll.), are important pests of potato. Plant parasitic nematodes produce multiple effector proteins, secreted from their stylets, to successfully infect their hosts. These include proteins delivered to the apoplast and to the host cytoplasm. A number of effectors from G. rostochiensis predicted to be delivered to the host cytoplas...

Field microplot experiments were conducted from 1987 to 1992 to determine the relationship between fresh weight leaf yield of shade tobacco (Nicotiana tabacum) and initial density of Globodera tabacum tabacum (encysted J2 per cm(3) soil). Initial nematode densities of 0.1 to 1,097 J2/cm(3) soil were negatively correlated with leaf yield, total shoot weight, and normalized plant height 5 to 6 we...

Nematode Collection and DNA Extraction Nematodes were collected following methods previously described [1]. Mixed-­‐stages of Anguina agrostis were obtained from infected bentgrass seed. Globodera ellingtonae eggs were obtained from cysts reared on potato in field microplots. A population of Xiphinema americanum originally obtained from a vineyard was cultured in soil on sudangrass in a greenho...

MANEVA, S. and Zl. TRIFONOVA, 2015. Globodera rostochiensis population density effect on potato growth and yield. Regression models estimation. Bulg. J. Agric. Sci., 21: 815–821 Effect of initial population densities (Pi) of Bulgarian population of Globodera rostochiensis on the population dynamics and potato plants growth and yield was investigated in glasshouse and estimated using mathematica...