The goal of the present study was to determine the physiologically relevant mechanisms for forskolin-induced relaxation of intact rat tail artery. We stimulated deendothelialized rat tail artery with phenylephrine and then relaxed the tissue with the addition of forskolin, a specific activator of adenylyl cyclase. We measured membrane potential with the use of microelectrodes, estimated intrace...

Forskolin is a diterpene that activates adenylate cyclase in a variety of mammalian cells. In addition of forskolin to blowfly salivary glands increased cyclic AMP accumulation and salivary secretion. There was a small increase in transepithelial movement of labelled Ca2+. Forskolin did not induce breakdown of labelled phosphatidylinositol or inhibit the stimulation of phosphatidylinositol brea...

Increased cAMP by stimulation of adenylyl cyclase with forskolin or by beta-adrenoceptor activation with isoproterenol increased phospholipase D (PLD) activity in tracheal smooth muscle strips. PLD activity was measured by the accumulation of phosphatidylethanol. A linear increase in the concentration of phosphatidylethanol was observed over 20 min in muscle strips treated with either forskolin...

We have studied the postnatal alterations of the neurotransmitter receptors in rat parotid gland, and found that the number of ƒÀ-adrenoceptors in the tissues and their affinity for the ƒÀ-agonists increased significantly during development and that these changes were coupled with amylase secretion by ƒÀ-agonist from the tissues. In this experiment, developmental changes in the adenylate cyclas...

Forskolin is one of the factors that activate adenylate cyclase, which increases the intracellular level of cyclic adenosine monophosphate (AMP). The growth inhibitory effect of forskolin on human glioma (KY) cells was examined both in vitro and in vivo. The cytotoxic effect was evaluated by colony formation assay, and the cytokinetic effect by deoxyribonucleic acid (DNA) histogra...

Forskolin is commonly used to stimulate adenylate cyclase in the study of modulation of ion channels and other proteins by adenosine 3',5'-monophosphate (cAMP)-dependent second messenger systems. In addition to its action on adenylate cyclase, forskolin directly alters the gating of a single class of voltage-dependent potassium channels from a clonal pheochromocytoma (PC12) cell line. This alte...

In this study, we present evidence on the ability of endogenous adenosine to modulate adenylyl cyclase activity in intact PC12 cells. The adenosine receptor antagonists PD 115199, xanthine amine congener, 8-cyclopentyl-1,3-dipropylxanthine, 8-(p-sulfophenyl)theophylline, and 3,7-dimethyl-1-propargylxanthine inhibited 10 microM forskolin-induced cyclic AMP (cAMP) accumulation, with IC(50) values...

In signal transduction research natural or synthetic molecules are commonly used to target a great variety of signaling proteins. For instance, forskolin, a diterpene activator of adenylate cyclase, has been widely used in cellular preparations to increase the intracellular cAMP level. However, it has been shown that forskolin directly inhibits some cloned K+ channels, which in excitable cells ...

Phosphorylation of phospholemman (PLM) on ser68 has been proposed to at least partially mediate cyclic AMP (cAMP) mediated relaxation of arterial smooth muscle. We evaluated the time course of the phosphorylation of phospholemman (PLM) on ser68, myosin regulatory light chains (MRLC) on ser19, and heat shock protein 20 (HSP20) on ser16 during a transient forskolin-induced relaxation of histamine...

Objectives: In patient-derived rectal organoids (PDRO), CFTR modulator efficacy can be evaluated in the forskolin-induced swelling (FIS) assay. this assay, PDRO response to acute stimulation with forskolin (Fsk) and potentiator(s) is quantified as a measure of restoration function after incubation corrector(s). However, high residual and/or (eg. potentiator effect elexacaftor), may swell alread...