Here, we used a fluorophore-bound, porphyrin-targeted DNA aptamer to increase the sensitivity of capillary electrophoresis for detection model porphyrin. The complex wit...

We designed a switchable fluorophore activated by UVA light and radical initiator, for optical lithography with concomitant metal-enhanced fluorescence silver nanoparticles.

the reaction of hydrogen peroxide and  pheny -9-acridinecarboxylate in the presence of rhodamin b as a fluorophore leads to the emission of either bright blue light  or bright red light, depending on the structure of fluorescer and reaction conditions. the light emitter responsible for the chemi luminescence (cl) reaction is acridone and that the blue light produced with out the fluorescers is ...

Over the past 15 years, fluorescence has become the dominant detection/sensing technology in medical diagnostics and biotechnology. Although fluorescence is a highly sensitive technique, where single molecules can readily be detected, there is still a drive for reduced detection limits. The detection of a fluorophore is usually limited by its quantum yield, autofluorescence of the samples and/o...

The electronic structure and photoinduced electron transfer processes in a K+ fluorescent sensor that comprises a 4-amino-naphthalimide derived fluorophore with a triazacryptand ligand is investigated using density functional theory (DFT) and time-dependent density functional theory (TDDFT) in order to rationalise the function of the sensor. The absorption and emission energies of the intense e...

Fluorescence guidance using 5-aminolevulinic acid (5-ALA) for brain tumor resection is a recent technique applied to the highly malignant brain tumors. Five-ALA accumulates as protoporphyrin IX fluorophore in the tumor cells in different concentrations depending on the tumor environment and cell properties. Our group has developed a fluorescence spectroscopy system used with a hand-held probe i...

Fluorescence double-labeling is an important method for investigating associations of molecules (1). For example, evidence of co-association of proteins often includes demonstrating that they are colocalized in the same compartment of the cell. Furthermore, colocalization studies are not limited to proteins but also include measuring the colocalization of separate lipid species (8), lipids with...

Spin-labeled lipids are commonly used as fluorescence quenchers in studies of membrane penetration of dye-labeled proteins and peptides using depth-dependent quenching. Accurate calculations of depth of the fluorophore rely on the use of several spin labels placed in the membrane at various positions. The depth of the quenchers (spin probes) has to be determined independently; however, experime...

Sub-surface tomography with diffuse light has been investigated with a non-contact approach to characterize the performance of optical absorption and fluorescence imaging. Using both simulations and experimental studies, the reconstructed images of local sub-surface heterogeneities was demonstrated, using diffusion properties characteristic of near-infrared light in mammalian tissue. The result...