An efficient protocol was established for rapid in vitro propagation of Stevia rebaudiana Bertoni, using explants from mature plants. The effect of 1/2MS, MS and B5 culture media, also 27 different combinations of auxins and cytokinins was studied on in vitro growth and development of Stevia rebaudiana Bertoni. The best culture medium for shoot and root formation was 1/2MS, producing 1.30 shoot...

We report combination of explants and enzymatic protocol as mixed enzymatic-explant procedure to faster extraction of MSCs from WJ. Umbilical cords (UC) were collected from Imam Khomini Hospital. For explant outgrowth, 6 9 pieces of WJ were transferred onto tissue culture flask and waited for attachment. For mixed enzymatic-explant, 1 cm pieces WJ were placed in enzymatic cocktail comprising 4 ...

An experiment was conducted to develop a suitable in vitro plant regeneration technique for Rosa hybrida L. cv. ‘Double Delight’ using nodal segments and leaf tissues as explants through direct indirect organogenesis. Aseptic were inoculated onto gelled MS medium contained various strengths of growth regulators alone combinations callus shoot induction. containing 2.0 mg/l BAP found be the most...

The influence of explant nodal positions on the in vitro shoot growth and proliferation were studied in the two rose cvs. ‘Bianca’ and ‘El Torro’. Third, fourth and fifth nodal explants were cultured on the modified MS medium supplemented with 1.0 and 5.0 mg/l BA. In both the cultivars, higher rate of proliferation (‘Bianca’ 3.75; ‘El Torro’ 2.65) were obtained from the explants distal to the a...

Background Currently, the use of stem cells from permanent (DPSCs) and deciduous teeth (SHEDs) has enabled many therapeutic advances. Despite numerous sources of stem cells, DPSCs and SHEDs have shown several advantages, since the isolation method is noninvasive and cells present rapid expansion in vitro [1]. DPSCs have shown the capacity to form a dentin-pulp like complex. Due to its high oste...

N -(Carboxymethyl)lysine (CML) over-stimulates choroidal neovascularization in vitro in streptozotocin (STZ)-diabetic rat. In this study, we investigated the effects of CML-induced choroidal angiogenic potential on retinal neovascularization during the course of STZ-induced diabetes in rats. Retinal and choroidal explants were isolated from the same eyeball of early-diabetic, advanced-diabetic ...

While there have been remarkable advances in hearing research over the past few decades, there is still no cure for Sensorineural Hearing Loss (SNHL), a condition that typically involves damage to or loss of the delicate mechanosensory structures of the inner ear. Sophisticated in vitro and ex vivo assays have emerged in recent years, enabling the screening of an increasing number of potentiall...

fig trees are threatened by the attack of fig mosaic disease (fmd) on leaves and fruits caused by viruses of several genera. shoot-tip culture is a convenient method for viral sanitation. for this purpose, a reliable protocol for rapid in vitro propagation was developed with shoot-tips of three major tunisian local fig (ficus carica l.) varieties zidi (zdi), soltani (sni), bither abiadh (ba) an...

Previous studies reported that hepatitis B virus (HBV) deoxyribonucleic acid (DNA) can be detected in livers of patients who received transplants for hepatitis B despite the absence of serological markers of HBV recurrence. Quantification of HBV DNA was not performed and presence of covalently closed circular (ccc) DNA was not analyzed in most studies. We aimed to quantify total and ccc HBV DNA...

the present work developed an efficient plant regeneration and propagation system via direct organogenesis for morinda officinalis how., a valuable endangered medicinal plant. the effects of explant types on shoot induction, plant growth regulators on shoot proliferationand elongation and the subsequent rooting ability of shoots were examined. among the various types of explant, direct shoot pr...