A protocol was developed for high frequency in vitro multiplication of an ornamental orchid, Phalaenopsis amabilis (L.) Blume var. Jawa, using plant growth regulators (PGRs). Protocorm-like bodies (PLBs), as explants were cultured on Murashige and Skoog (MS) medium fortified with various concentrations of kinetin (KIN) and indole-3-butyric acid (IBA), either individually or in...

A micropropagation protocol was developed which may assist in the safeguarding and augmentation of dwindling natural populations of Clinopodium odorum (Griseb.) Harley, a critically and endangered medicinal plant. Factors affecting culture initiation bud sprouting and growth, rooting, and acclimatization were studied, using nodal segments of in vitro germinated seedling as primary explants on s...

An efficient and reproducible procedure was established for direct shoot regeneration of Solanum surattense Bum. using internodal explants. The shoot regeneration efficiency on Murashige and Skoog (MS) medium supplemented with 6benzylaminopurine (BAP) was superior in compared to 6furfurylaminopurine (Kn). The highest mean number of shoots per explant was recorded on MS medium containing 0.5 mgl...

Abstract Kale ( Brassica oleracea convar. acephala var. sabellica ) is one of the oldest vegetable varieties with highest nutritional value and stress resistance. This work aimed to establish an efficient kale micropropagation protocol. Shoot tips were used as explants during axillary shoots multiplication. The most effective cytokinin (6 10 per explant after 1st 2nd passage, respectively) was ...

Plants propagated by seed do not ensure genetic uniformity and are sometimes infected with diseases. In Vitro micropropagation techniques an alternative method to traditional cloning approaches for producing true-to-type pathogen-free plants. However, due the particularities of in vitro environment, these plants face many challenges, often critical their survival, adapt ex conditions. this cont...

Brassavola nodosa (L.) Lindl. is a tropical epiphytic orchid showing characteristics of interest for the ornamental nursery industry, as well considered an endangered species. Problems with traditional propagation methods limit development large-scale commercial production system. The use micropropagation has been investigated this species, and liquid in vitro systems showed potential temporary...

This study investigated the effects of different PGRs on in vitro plant regeneration Anthurium scherzerianum Schott and acclimatization ex stage. Depending objective each experiment, shoots were induced from callus cultured a modified Murashige Skoog (MS) medium containing cytokinins auxins. Results showed that 90% leaf explants 48% petiole produced callus. A half-strength MS supplemented with ...