A simple and highly sensitive homogeneous aptasensor is developed, which relies on nicking enzyme. The sensitivity of this newly proposed aptasensor is about three orders of magnitude higher than that of traditional homogeneous aptasensors. Furthermore, it is capable of detecting target protein in real samples.

The discovery of the DNA-mediated assembly of gold nanoparticles was a great moment in the history of science; this understanding and chemical control enabled the rational design of functional nanomaterials as novel probes in biodetection. In contrast with conventional probes such as organic dyes, gold nanoparticles exhibit high photostability and unique size-dependent optical properties. Becau...

A previously undescribed monopartite begomovirus was identified in Kampot province, Cambodia, Malvastrum coromandelianum plants exhibiting yellow vein symptoms characteristic of infections. The apparently full-length viral component cloned and sequenced following enrichment circular DNA by rolling-circle amplification restriction enzyme digestion. genome the virus 2737 nucleotides length (KP188...

In this paper the operation of an amperometric biosensor producing a chemically amplified signal is modelled numerically. The chemical amplification is achieved by using synergistic substrates. The model is based on non-stationary reaction-diffusion equations. The model involves three layers (compartments): a layer of enzyme solution entrapped on the electrode surface, a dialysis membrane cover...

We developed a simple, rapid and reliable method to delete DNA fragments in plasmids using a polymerase chain reaction based amplification of the circular DNA sequence that excludes the fragment to be deleted. The primers are designed to contain a non-complementary 5' sequence consisting of a restriction enzyme target sequence. Following PCR amplification, the plasmid is digested with Dpn I to ...

We report the first use of a polymerization-based ELISA substrate solution employing enzymatically mediated radical polymerization as a dual-mode amplification strategy. Enzymes are selectively coupled to surfaces to generate radicals that subsequently lead to polymerization-based amplification (PBA) and biodetection. Sensitivity and amplification of the polymerization-based detection system we...

Ribonucleic acid (RNA) quantification is an essential technique in biology. There has been remarkable progress RNA techniques over the recent years; however, specificity of these to quantify a very small amount doubtful because factors which can inhibit precise quantification. To develop that leads most sensitive quantification, problems must be overcome. In this article, we first review RNA: q...