We used Salmonella enteritidis serotype dublin strain SL1438, a nonreverting, aromatic-dependent, histidine-requiring mutant, as a recipient for a recombinant plasmid coding for production of the nontoxic B subunit of the heat-labile Escherichia coli enterotoxin. The S. enteritidis derivative EL23 produced heat-labile enterotoxin subunit B that was indistinguishable from heat-labile enterotoxin...

The cross-reactivity of monoclonal antibodies produced against staphylococcal enterotoxin A with purified and crude enterotoxins B, C1, D, and E and the specificity of such reactions were evaluated by the indirect enzyme-linked immunosorbent assay and immunoblotting of Western blots (from sodium dodecyl sulfate-polyacrylamide gel electrophoresis) followed by autoradiography. Purified and crude ...

the present study was aimed to identify the frequency of coagulase (coa) and thermonuclease (nuc) genes and staphylococcal enterotoxin a (sea) production among staphylococcus aureus isolated from various sources in shiraz. moreover, the correlation between the sea gene and coagulase and thermonuclease enzymes is also considered. a total of 100 s. aureus were isolated from various sources includ...

The prevalence of the hemolytic enterotoxin complex HBL was determined in all species of the Bacillus cereus group with the exception of Bacillus anthracis. hblA, encoding the binding subunit B, was detected by PCR and Southern analysis and was confirmed by partial sequencing of 18 strains. The sequences formed two clusters, one including B. cereus and Bacillus thuringiensis strains and the oth...

Cholera-like enterotoxin produced by a non-O1 strain of Vibrio cholerae, S7 (S7 enterotoxin), isolated from human diarrheal stool, was purified, and its physicochemical, biological, and immunological properties were compared with those of cholera enterotoxin from V. cholerae O1 569B (CT) and an enterotoxin produced by another non-O1 V. cholerae (E8498 enterotoxin) reported previously (Yamamoto ...

conclusions the presence of bft gene in b. fragilis isolates wouldnot certainly result in diarrheaamong patients. there is not enough accordance between ddm and swedish e tests for antimicrobial susceptibility of b. fragilis for some antibiotics, although in other cases a good agreement was observed. background enterotoxigenic bacteroides fragilis (etbf) are one of the most important anaerobic ...

background and objectives:   staphylococcus aureus is one of the main causatives of bovine mastitis. resistance of some strains to methicillin, can complicate the treatment of its infections. on the other hand, enterotoxin production is also im- portant. therefore, the aim of our study was to investigate the methicillin resistance and enterotoxin production in s. aureus isolates caused bovine m...

A rapid multiplex PCR approach was developed to detect the bft gene subtypes in Bacteroides fragilis clinical isolates. This technique could be used to look at the epidemiology of enterotoxigenic strains of B. fragilis in clinical infections and whether there is a correlation between disease and the presence of B. fragilis enterotoxin.