D uring mammalian tooth formation, two proteinases are secreted by ameloblasts: enamelysin (MMP-20) and kallikrein-4 (KLK4). Enamelysin is the early protease. It is expressed by ameloblasts throughout the secretory stage and part of the maturation stage. KLK4 is the late protease; its expression by ameloblasts begins in the transition stage and continues throughout enamel maturation. 5 Expressi...

D uring mammalian tooth formation, two proteinases are secreted by ameloblasts: enamelysin (MMP-20) and kallikrein-4 (KLK4). Enamelysin is the early protease. It is expressed by ameloblasts throughout the secretory stage and part of the maturation stage. KLK4 is the late protease; its expression by ameloblasts begins in the transition stage and continues throughout enamel maturation. 5 Expressi...

BACKGROUND Mutations in WDR72 have been identified in autosomal recessive hypomaturation amelogenesis imperfecta (AI). OBJECTIVE to describe a novel WDR72 mutation and report the ultrastructural enamel phenotype associated with a different WDR72 mutation. METHODS A family segregating autosomal recessive hypomaturation AI was recruited, genomic DNA obtained and WDR72 sequenced. Four deciduou...

Mice lacking amelogenin (KO) have hypoplastic enamel. Overexpression of the most abundant amelogenin splice variant M180 and LRAP transgenes can substantially improve KO enamel, but only ~40% of the incisor thickness is recovered and the prisms are not as tightly woven as in WT enamel. This implies that the compositional complexity of the enamel matrix is required for different aspects of ename...

enamel matrix derivative emdogain (emd) is widely used in periodontal treatment in spite of the fact that its effect on the developing embryo has not been elucidated. the aim of this study was to investigate the teratogenic effect of emd on the rat embryo neural crest cells. the neural crest is a unique population of cells that migrates from the dorsal neural tube along defined pathways and pro...

Dentin matrix protein 1 (DMP1) is an acidic non-collagenous protein that is necessary for the proper biomineralization of bone, cartilage, cementum, dentin, and enamel. Dentin matrix protein 1 is highly phosphorylated and potentially glycosylated, but there is no experimental data identifying which specific amino acids are modified. For the purpose of facilitating the characterization of DMP1 f...

  Objective: At present, growth factor-containing products such as enamel matrix derivatives, recombinant bone morphogenetic protein (rh-BMP), recombinant platelet derived growth factor and platelet rich plasma (PRP) have gained increasing attention. PRP is an autologous source of platelet growth factors used to enhance healing of soft and hard tissues. PRP has gained popularity due to its auto...

The hardest tooth enamel tissue develops from a soft layer of protein-rich matrix, predominated by amelogenin that is secreted by epithelial ameloblasts in the secretory stage of tooth enamel development. During enamel formation, a well-controlled progressive removal of matrix proteins by resident proteases, Matrix metalloproteinase 20 (MMP20), and kallikrein 4 (KLK4), will provide space for th...

objective: at present, growth factor-containing products such as enamel matrix derivatives, recombinant bone morphogenetic protein (rh-bmp), recombinant platelet derived growth factor and platelet rich plasma (prp) have gained increasing attention. prp is an autologous source of platelet growth factors used to enhance healing of soft and hard tissues. prp has gained popularity due to its autolo...