Neutral salts exhibit very marked differences as eluants of proteins from affinity columns. We observe: (i) that the relative potencies of neutral salts as eluants are independent of the protein or the affinity ligand in the systems studied, (ii) that the absolute salt concentration necessary to elute any given protein bound to the affinity matrix is proportional to the algebraic sum of a set o...

The rates of elution from RBC of the Lee and PR8 strains of influenza virus were studied by means of a step-wise elution technique. By means of a single treatment with lanthanum acetate or irradiation with ultraviolet and subsequent passage in chick embryos, it was possible to alter the elution rate of the Lee strain so that it was similar to that of the PR8 strain. This alteration proved to be...

In order to reveal the influence of ammonium salts on rare earth leaching process weathered crust elution-deposited ores, acetate, chloride, and sulfate were used as agents. The effects agent efficiency expansion, dissolution, transformation behavior clay minerals in studied. results showed that followed acetate > chloride sulfate, with values 90.60%, 85.96%, 84.12%, respectively. swelling r...

An in-line monitoring method for the elution process of Ginkgo biloba L. leaves using visible and near-infrared spectroscopy in conjunction with multivariate statistical control (MSPC) was established. Experiments, including normal operating batches abnormal ones, were designed carried out. The MSPC model developed validated. abnormalities detected successfully by charts principal component sco...

a solid-phase extraction (spe) method for sample clean-up and preconcentration followed by reversed-phase high-performance liquid chromatography (rp-hplc) with uv detection is reported for determination of polycyclic aromatic hydrocarbons (pahs) in the caspian seawater. a good resolution was obtained using acetonitrile:water (40:60) as mobile-phase for separation of these compounds. the effect ...

Affinity purification of proteins using antibodies coupled to beads and subsequent mass spectrometric analysis has become a standard technique for the identification of protein complexes. With the recent transfer of the isotope dilution mass spectrometry principle (IDMS) to the field of proteomics, quantitative analyses-such as the stoichiometry determination of protein complexes-have become ac...

BACKGROUND Antibiotic bone cement is commonly used in staged revision arthroplasty as well as the treatment of open fractures. Multiple factors affect antibiotic elution from bone cement. This study was performed to investigate the effect of two variables, the quantity of liquid monomer and the timing of antibiotic addition, on the ultimate elution of antibiotic from bone cement. METHODS Vanc...

This article details the rapid extraction of amphetamines from oral fluid using low specimen volume, low sorbent bed mass, and fast gas chromatography with mass selective detection. The collection of a known amount of sample volume coupled with high percentage recovery of drug from the collection pad has allowed oral fluid to be increasingly employed as the specimen of choice for the detection ...

Protein behavior in Hydrophobic Interaction Chromatography using different chromatographic conditions was investigated. A linear correlation was found between protein retention time on different matrixes and different initial elution salt concentrations. Mathematical correlations between retention times under different chromatographic conditions were obtained and validated, which can be used in...