نتایج جستجو برای: elisa kits respected to pnrsv prunus necrotic ring spot virus
تعداد نتایج: 10842838 فیلتر نتایج به سال:
Background and Aims: In spite of a long tradition of fruit-tree growing in all provinces of Iran, information on pear (Pyrus communis) tree viruses is scant. In order to identify of the nature of the virus like symptoms and decline in pear trees, we carried out a survey for the occurrence of four important viruses. Materials and Methods: Leaf samples of pear showing virus like symptoms wer...
background and aims: in spite of a long tradition of fruit-tree growing in all provinces of iran, information on pear (pyrus communis) tree viruses is scant. in order to identify of the nature of the virus like symptoms and decline in pear trees, we carried out a survey for the occurrence of four important viruses. materials and methods: leaf samples of pear showing virus like symptoms were col...
abstract iris yellow spot virus (iysv) is one of the important pathogens of onion in reported countries. host range of this virus is very wide and transmitted by onion thrips (thrips tabaci). in survey that was conducted in summer 2006 in onion fields of mashhad, samples with viral symptoms and high population of thrips have been collected. since the symptoms were similar to iysv, special antis...
In New Zealand, an arbovirus surveillance program has been operating for more than 20 years, which includes testing of cattle with the Akabane virus neutralization test. With the aim to replace this laborious test by an easier-to-perform enzyme-linked immunosorbent assay (ELISA), 2 commercial ELISA kits, ELISA-1 from France (originally from Australia) and ELISA-2 from Japan, were compared, usin...
abstract polymyxa betae is an obligate parasite and has a worldwide distribution. this fungus is a soil-borne root endoparasite in some plant families such as portulaceae, chenopodiaceae and amaranthaceae. this fungus is the vector of many plant viruses such as beet necrotic yellow vein virus (bnyvv) which causes the disease known as rhizomania. bnyvv is the most economical important viral dise...
carnation (dianthus caryophyllus) can be infected by several viruses that some are specific to carnation flowers. vegetative propagation of carnation, can easily disperse viruses by infectied cuttings. during the year 2007, viral-like symptoms were observed on carnation in mahallat greenhouses. 142 suspected samples from glass houses, with symptoms of reddish- purple flecks and spots on leaves,...
ویروس لکه حلقوی بافت مرده هسته دارها (prunus necrotic ringspot virus, pnrsv)، یکی ازمخربترین ویروسهای گیاهی است که میتواند باعث کاهش رشد و عملکرد در درختان میوه هسته دار شود. در این مطالعه شیوع این بیماری در درختان بادام با هدف شناسایی مطمئن این ویروس با دو روش rt-pcr و الایزا در باغ های بادام منطقه ی سامان در استان چهارمحال و بختیاری و تاثیر آن بر جوانه زنی دانه گرده بررسی شد. 100 درخت...
A one-step reverse transcription-polymerase chain reaction (RT-PCR) procedure was developed to detect Cherry green ring mottle virus (CGRMV) and Cherry necrotic rusty mottle virus (CNRMV) in woody indicators and naturally infected Prunus spp. Viral RNA suitable for RT-PCR was obtained by a simple trapping method that did not require either extraction of double-stranded RNA (dsRNA) or total RNA,...
شمار 149 نمونۀ برگی از درختان میوۀ هستهدار و دانهدار استان کردستان هنگام بهار و تابستان سالهای 1394 و 1396 بهمنظور شناسایی مولکولی و بررسی گوناگونی ژنتیکی عامل لکه حلقوی بافت مردۀ درختان هستهدار جمعآوری شدند و با آغازگرهای اختصاصی ژن پروتئین پوششی PNRSV-F3/PNRSV-R3 آزمون RT-PCR شدند. نتیجهها RT-PCR نشان دادند که 8/20 درصد از نمونهها آلوده به PNRSV بودند. سیزده جدایه بر پایۀ نوع میزبان و...
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