نتایج جستجو برای: electro elution

تعداد نتایج: 27067  

Journal: :The Analyst 2012
Ilaria De Santo Luigi Sanguigno Filippo Causa Tullio Monetta Paolo A Netti

Drug elution properties of TiO(2) nanotube arrays have been largely investigated by means of solely macroscopic observations. Controversial elution performances have been reported so far and a clear comprehension of these phenomena is still missing as a consequence of a lack of molecular investigation methods. Here we propose a way to discern drug elution properties of nanotubes through the eva...

Journal: :The Journal of bone and joint surgery. British volume 2011
B A Rogers F R Middleton N Shearwood-Porter S Kinch A Roques N W Bradley M Browne

Two-stage revision surgery for infected total knee replacement offers the highest rate of success for the elimination of infection. The use of articulating antibiotic-laden cement spacers during the first stage to eradicate infection also allows protection of the soft tissues against excessive scarring and stiffness. We have investigated the effect of cyclical loading of cement spacers on the e...

Journal: :The Journal of biological chemistry 1976
I M Chaiken H C Taylor

Quantitative affinity chromatography on uridine-5'-(Sepharose-4-aminophenylphosphoryl)-2'(3')-phosphate was developed for the study of binding of ribonuclease species to nucleotide ligands. Elution of the native species ribonuclease-A and -S on the afffinity matrix in 0.4 M ammonium acetate, pH 5.2, containing various amounts of the soluble competing ligand 2'-cytidine monophosphate, reveals an...

2015
Marek Leśko Dennis Åsberg Martin Enmark Jörgen Samuelsson Torgny Fornstedt Krzysztof Kaczmarski

The inverse method is a numerical method for fast estimation of adsorption isotherm parameters directly from a few overloaded elution profiles and it was recently extended to adsorption isotherm acquisition in gradient elution conditions. However, the inverse method in gradient elution is cumbersome due to the complex adsorption isotherm models found in gradient elution. In this case, physicoch...

Journal: :BioTechniques 2013
John LaCava Nagarajan Chandramouli Hua Jiang Michael P Rout

Here we report a modified peptide reagent useful for the rapid, native elution of protein complexes containing a Protein-A-tagged component. We tested this reagent for the elution of tagged endogenous protein complexes from yeast (Nup53p/Nup170p dimer; Nup1p/Kap95p/Kap60p trimer; pentameric GINS complex) and bacteria (RNAP holoenzyme). The majority of the affinity-isolated material is released ...

Journal: :iranian journal of environmental sciences 0
ali vosoogh phd candidate in civil-water engineering, iran university of science & technology, iran akbar baghvand associate professor, faculty of environment, university of tehran, iran hatef saghakhaneh msc. student of civil engineering-environment, aras international campus, university of tehran, iran

groundwater makes up an important part of global freshwater resources, though it is often threatened by overuse of natural resources along with abundant production of wastes in modern society. this study aims to investigate the removal of three heavy metals including nickel, cadmium, and lead from groundwater via electrocoagulation, a suitable method for treatment of water-soluble compounds, de...

Journal: :Veterinary surgery : VS 2003
Jose R Ramos Rick D Howard R Scott Pleasant H David Moll Dennis J Blodgett Geraldine Magnin Thomas J Inzana

OBJECTIVE To characterize the elution and bioactivity of metronidazole and gentamicin sulfate polymerized, individually and in combination, with polymethylmethacrylate (PMMA). STUDY DESIGN In vitro experimental study. METHODS PMMA beads containing metronidazole (3 concentrations), gentamicin sulfate, or metronidazole and gentamicin sulfate were immersed in 5 mL of phosphate-buffered saline ...

Journal: :Journal of chromatography. A 2006
Adam P Schellinger Peter W Carr

Chromatographers are cautioned to avoid gradient elution when isocratic elution will do. In this work, we compared the analytical properties of gradient and isocratic separations of a sample which can be done quite readily under isocratic conditions. We found that gradient elution gave a shorter overall analysis with similar resolution of the critical pair compared to isocratic elution without ...

Journal: :Analytical biochemistry 1968
J K Smith D W Moss

paratus is the dimension of the elution cham ber; if a large chamber is employed, dilution of solute occurs thus resulting in broadening of the elution patterns, while with a narrow chamber elution is frequently interrupted by the swelling of gel during operation. In many cases, either upward or downward elution has been employed and this leads to com plication of the apparatus. This communi ca...

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