Clinical pathology testing of rodents is often challenging due to insufficient sample volume. One solution in clinical veterinary and exploratory research environments is dilution of samples prior to analysis. However, published information on the impact of preanalytical sample dilution on rodent biochemical data is incomplete. The objective of this study was to evaluate the effects of preanaly...

Until now, limited observational data have suggested that the juxtaposition of trees with respect to place where a GPS position fix is collected may affect static horizontal position accuracy of that determined position. Our goal was to assess GPS accuracy with respect to the spatial arrangement of nearby trees, and determine whether correlations existed or whether trends were evident. Therefor...

Objective: Mammalian spermatozoa swell under hypotonic conditions. The exposure of spermatozoa to isotonic conditions after exposure to hypertonic conditions during cryopreservation results in the occurrence of osmotic shock, which is characterized by coiling of the distal end of the sperm tail. The objective of this study was to assess the effects of osmotic shock (% sperm coiling) on human cr...

The virus content of naturally occurring and experimentally induced papillomas of cottontail rabbits varies considerably. As a rule different individual papillomas on the same rabbit were found to have a similar virus content. The amount of virus usually increased as the tumors aged from 6 to 12 weeks. After 12 weeks there was no consistent pattern of change in virus con tent of papillomas, and...

A series of alkene bearing small molecules were electrografted to the surface carbon fibers using in situ generation aryldiazonium salts. These used as an anchoring point for use Ring Opening Metathesis Polymerization (ROMP) a ‘graft from’ approach fiber modification. After modification, evaluation these modified epoxy resin, single fragmentation test, showed significant increases interfacial s...

A number of hydrothermal vent sites exist on the summit of the Loihi Seamount, a shield volcano that is part of the Hawaiian archipelago. The vents are 1,100 to 1,325 m below the surface and range in temperature from slightly above ambient (10 degrees C) to high temperature (167 degrees C). The vent fluid is characterized by high concentrations of CO2 (up to 17 mM) and Fe(II) (up to 268 microM)...

Human KC cell monolayer inoculated with concentraten Mason-Pfizer monkey virus (MPMV) showed syncytia formation within an hour. The cell fusion was blocked by the treatment of the MPMV with neutralizing antiserum. Treatment of the MPVM with beta-propiolactone resulted in the loss of infectivity although KC cell fusion ability of the virus still remained. KC cells inoculated with unconcentrated ...

The stem-cell response of the Lewis lung carcinoma to single doses of cyclophosphamide has been studied by three assay techniques: in vitro colony formation, lung colony formation, and the end-point dilution assay. These three techniques have given comparable results, and the end-point dilution results showed that 50 percent takes could be achieved with as few as 1 to 3 cells. Studies have been...

We have developed a novel method for urine culture for office practice based on the use of filter paper as a solid-phase dilution device. Filtration dilutes and spreads the inoculum onto a solid culture surface. Experiments were conducted to determine the optimum inoculum size, microbial permeability through filter papers, and ability to exclude vaginal epithelial cells. The filter paper diluti...

Phagocytic cells such as neutrophils and macrophages are potential components of the immune defense that protects mammals against Candida albicans infection. We have tested the interaction between the mouse macrophage cell line RAW 264.7 and a variety of mutant strains of C. albicans. We used an end point dilution assay to monitor the killing of C. albicans at low multiplicities of infection (M...