The methylation of Escherichia coli B transfer RNA in vitro by HeLa mitochondrial and cytoplasmic extracts was examined. The following differences were found. (a) The cytoplasmic extract methylated E. coli B tRNA to a much greater extent than did the mitochondrial extract. (b) The two extracts methylated different sites on E. coli B tRNA, thereby generating different methylated oligonucleotide ...

The methylation of Escherichia coli B transfer RNA in vitro by HeLa mitochondrial and cytoplasmic extracts was examined. The following differences were found. (a) The cytoplasmic extract methylated E. coli B tRNA to a much greater extent than did the mitochondrial extract. (b) The two extracts methylated different sites on E. coli B tRNA, thereby generating different methylated oligonucleotide ...

Vitis vinifera L.is a traditional Asian herb widely used for different health problems. In the present research, the ethanolic and the aqueous extracts of Vitis vinifera L. leaves collected from shrub, grown in Tunisia, were prepared and evaluated for the antileishmanial activity against Leishmani ainfantum promastigotes. The inhibitory concentration 50 (IC50) was determined and the results sho...

This article briefly reviews the classical cell cycle studies using oocytes and zygotes of mainly amphibians in the past century. The discussions are focused on the investigations into the cytoplasmic factors that regulate meiosis during oocyte maturation and the initiation of mitosis during fertilisation, which were carried out in the author's lab between 1967 and 1987. This chronicle traces t...

background : many studies have investigated the effects of psychological factors on disease activity in patients with ulcerative colitis (uc). however, the number of studies that have assessed the correlation between immunologic markers of uc and psychological distress are few. therefore, we designed a study to determine the association between two immuno-biological markers, peripheral/central ...

this study was designed to evaluate the detrimental effect of atrazine (atr) on germinal epitheliums (ge) cytoplasmic carbohydrate (ch) and unsaturated fatty acids (ufa) ratio and to clarify the effect of atr on serum levels of fsh, lh, testosterone and inhibin-b (inh-b). the impact of atr exposure on total antioxidant capacity (tac), sperm dna packing and integrity were also investigated. seve...

Purified ribonucleoprotein complexes of human parainfluenza virus type 3 (HPIV-3) virions required, in addition to the viral proteins, soluble cytoplasmic proteins from uninfected cells for the synthesis of mRNAs in vitro. In contrast to Sendai virus transcription, in vitro RNA synthesis from HPIV-3 ribonucleoprotein complexes was not stimulated significantly by purified tubulin. Moreover, cyto...

Introduction and objective: Esterase activity is used in evaluation and correlation of strains in fungi. Our previous study showed that Candida albicans has a kind of intracellular esterase activity in yeast extract, peptone, and glucose medium (YPG). The aim of this research was to study the qualitative and quantitative differences of this enzymatic activity among clinical isolates of this yea...

A particle-free extract has been prepared from rat liver which is capable of incorporating isotopic acetate, pyruvate, and acetyl CoAl into long chain fatty acids. While both cytoplasmic and mitochondrial enzymes are necessary for the complete system, evidence is presented suggesting that the presence of electrolyt,es in the homogenizing medium may act to extract the lipogenic enzymes of the mi...

A particle-free extract has been prepared from rat liver which is capable of incorporating isotopic acetate, pyruvate, and acetyl CoAl into long chain fatty acids. While both cytoplasmic and mitochondrial enzymes are necessary for the complete system, evidence is presented suggesting that the presence of electrolyt,es in the homogenizing medium may act to extract the lipogenic enzymes of the mi...