Wild-type cytochrome P450 CYP102A1 from Bacillus megaterium is a highly efficient monooxygenase for the oxidation of long-chain fatty acids. The unique features CYP102A1, such as high catalytic activity, expression yield, regio- and stereoselectivity, self-sufficiency in electron transfer fusion protein, afford requirements an ideal biocatalyst. In past three decades, remarkable progress has be...

The role of endogenous arachidonic acid and its metabolites as mediators of cell growth was studied in rat mesangial cells. Inhibitors of the cytochrome P450 monooxygenase and lipoxygenase systems (nordihydroguaiaretic acid (NDGA), SK&F 525A, and ketoconazole) significantly reduced serum-stimulated cell growth as determined by cell counts and incorporation of [3H]thymidine. Inhibition of cycloo...

The cytochrome P450 monooxygenase (P450) enzyme system is a major mechanism of xenobiotic biotransformation. The nicotinamide adenine dinucleotide phosphate (NADPH)-cytochrome P450 reductase (CPR) is required for transfer of electrons from NADPH to P450. One CPR gene was identified in the genome of the malaria-transmitting mosquito Anopheles stephensi Liston (Diptera: Culicidae). The gene encod...

The monooxygenase P450 BM-3 is a NADPH-dependent fatty acid hydroxylase enzyme isolated from soil bacterium Bacillus megaterium. As a pivotal member of cytochrome P450 superfamily, it has been intensely studied for the comprehension of structure-dynamics-function relationships in this class of enzymes. In addition, due to its peculiar properties, it is also a promising enzyme for biochemical an...

In the hematopoietic system the adherent stromal cells produce cytokines necessary for proliferation and differentiation of hematopoietic cells. In the present study, we showed the ability of adherent stromal cells to generate novel metabolites of arachidonic acid via the NADPH-cytochrome P450-dependent monooxygenase system. These metabolites were recovered in the incubation media, suggesting t...

Cinnamophilin (CIN) showed protective effect against oxidative damage. Cytochrome P450-dependent monooxygenase (P450) and glutathione S-transferase (GST) are important drug-metabolizing enzymes and P450 plays an important role in drug-drug interactions. To assess the possible drug interactions, effects of CIN on mouse P450 and GST were studied. In vitro, CIN inhibited liver microsomal nifedipin...

The increasing needs of personalized therapies have recently stimulated impressive advances in research and development of portable point-of-care biosensors for realtime monitoring as well as biosensors for drug development [1, 2]. Enzymatic amperometric biosensors based on the enzyme family cytochrome P450 (CYP) have drawn increasing attention because CYP is a monooxygenase enzyme primarily in...

Antipsychotics (APs) are psychotropic drugs that generally have a psycholeptic effect, capable of reducing psychotic symptoms and psychomotor agitation. This class is widely used in psychiatric practice, especially for the treatment psychosis schizophrenia other disorders. Most APs pass through biotransformation process, or metabolism, after they enter body before being eliminated. There three ...