A bone marrow transplant (BMT) is one kind of standard treatment modality in advanced hemato-oncology. In order to set up a BMT unit, one of the important steps before starting a clinical program is to evaluate the cryopreservation procedure for stem cell storage. Twenty one bags of buffy coat were used to be the testing specimens. They were processed and frozen according to cryopreservation pr...

Effective cryopreservation of oocytes is critically needed in many areas of human reproductive medicine and basic science, such as stem cell research. Currently, oocyte cryopreservation has a low success rate. The goal of this study was to understand the mechanisms associated with oocyte cryopreservation through biophysical means using a mouse model. Specifically, we experimentally investigated...

OBJECTIVE To evaluate the effect of magnetic-activated cell sorting in cryopreservation-thawing protocols on sperm motility and cryosurvival rate. DESIGN Prospective-controlled study. SETTING Andrology department at a university-based medical institution. PATIENT(S) Ten healthy volunteer sperm donors. INTERVENTION(S) Sperm populations were separated using annexin-V magnetic-activated ce...

OBJECTIVE Comparison of time intervals from diagnosis to chemotherapy between patients opting for embryo cryopreservation or ovarian tissue cryopreservation. STUDY DESIGN Retrospective analysis. SETTING University hospital in the Netherlands. PATIENTS AND METHODS Thirty-five female patients undergoing fertility preservation procedures before treatment with chemotherapy for cancer. Embryo ...

Protocorm-like bodies (PLBs) of Brassidium Shooting Star orchid were successfully cryopreserved using droplet-vitrification method. Vitrification based cryopreservation protocol is comprised of preculture, osmoprotection, cryoprotection, cooling, rewarming, and growth recovery and each and every step contributes to the achievement of successful cryopreservation. In order to reveal the lethal an...

There is growing concern over the effect of sperm cryopreservation on DNA integrity and the subsequent development of offspring generated from this cryopreserved material. In the present study, membrane integrity and DNA stability of Xenopus laevis and Xenopus tropicalis spermatozoa were evaluated in response to cryopreservation with or without activation, a process that happens upon exposure t...

Background: Cancer treatment methods can lead to male infertility .in this regard, cryopreservation of spermatogonial stem cells (SSC) and cell-to-person transplantation after the course of treatment to resolve the problem of infertility is a good one. The cryopreservation of SSC is an important process as it can help on the return of spermatogenesis. However, during this process, the stem cell...

PURPOSE We review the history of the cryopreservation of human sperm. The current methods of cryopreservation and indications are highlighted, and the success rates of the various uses of cryopreserved sperm are reviewed. Potential adverse sequelae of human cryopreservation are also discussed. MATERIALS AND METHODS Indications for cryopreservation include several applications, namely homologo...

OBJECTIVE To evaluate cryodamage on human sperm motility, cryosurvival rate, and sperm chromatin assessed by acridine orange staining method (AO test) after a six-month freeze-thawing process using liquid nitrogen vapor. STUDY DESIGN Experimental study. MATERIAL AND METHOD Twenty normal semen samples were obtained from the male partner of infertile couples attending the infertility unit, Si...

Cryopreservation of human spermatozoa has been used since 1954 (1). After surgical treatment, chemotherapy, or radiotherapy, permanent sterility or transient infertility of up to 5 years can occur (2). Therefore, cryopreservation is often recommended to preserve future fertility. However, sperm survival and motility are known to significantly decrease after the freeze-thaw process. Since sperm ...