نتایج جستجو برای: complementary dna cdna
تعداد نتایج: 593175 فیلتر نتایج به سال:
PURPOSE The adult newt can regenerate lens from pigmented epithelial cells (PECs) of the dorsal iris via dedifferentiation. The purpose of this research is to obtain sequence resources for a newt lens regeneration study and to obtain insights of dedifferentiation at the molecular level. METHODS mRNA was purified from iris during dedifferentiation and its cDNA library was constructed. From the...
Although epidemiological observations, IgG passive transfer studies and experimental infections in humans all support the feasibility of developing highly effective malaria vaccines, the precise antigens that induce protective immunity remain uncertain. Here, we review the methodologies applied to vaccine candidate discovery for Plasmodium falciparum malaria from the pre- to post-genomic era. P...
In this context, in the age of genomic sciences, sequencing the entire genome or portion of it, can be thought as the straightforward step to understand a given venom composition. Particularly because, in many cases, the venom is produced in so small quantities, requiring great challenge (natural and bureaucratic) to obtain biological material for its investigation or the necessity of sacrifice...
The sequence presented was obtained by screening a cDNA library constructed in lambda gtlO from human mammary carcinoma with probes derived from cDNA of the translationally controlled, growth-related mouse tumor protein p23 (1,2). It has 86% similarity to the mouse cDNA sequence and encodes a protein with 96% similarity to the mouse tumor protein. The 3-primed noncoding region of the appropriat...
We have been conducting a mouse cDNA project to predict protein-coding sequences of mouse homologues of human KIAA and FLJ genes since 2001. As an extension of these projects, we herein present the entire sequences of 500 mKIAA cDNA clones and 4 novel cDNA clones that were incidentally identified during this project. We have isolated cDNA clones from the size-fractionated mouse cDNA libraries d...
Complementary DNAs to rat ventral prostate poly(A) RNA were cloned into pBR322 by the “dG-dC tailing” procedure. Clones containing cDNAs to the mRNAs coding for each of the three subunits of a major secretory protein (prostatein) were identified by hybridarrested translation. A 457-nucleotide base pair cDNA (E45) and a portion of a 365-base pair cDNA (E85) were analyzed to determine the composi...
We have postulated that the catecholamine-synthesizing enzymes tyrosine hydroxylase (TH), dopamine beta-hydroxylase (DBH), and phenylethanolamine N-methyltransferase (PNMT) are coded for by similar genes. To analyze the structural relationship of genes coding for these enzymes, we have cloned DNAs complementary (cDNA) to DBH and PNMT messenger RNAs (mRNAs). Using hybrid selection analysis to id...
α1,3Galactosyltransferase (α1,3GT) synthesizes α-gal epitopes (Galα1-3Galβ1-4GlcNAc-R) on glycoconjugates in nonprimate mammals but not in humans. Transduction of α1,3GT gene into human HeLa cells by an adenovirus vector allowed for accurate kinetics studies on the appearance of α1,3GT and of its product, the α-gal epitope, in the transduced cells. Mouse α1,3GT cDNA was inserted into a replicat...
Whole-genome sequencing is fundamental to understanding the genetic composition of an organism. Given the size and complexity of the soybean genome, an alternative approach is targeted random-gene sequencing, which provides an immediate and productive method of gene discovery. In this study, more than 120000 soybean expressed sequence tags (ESTs) generated from more than 50 cDNA libraries were ...
Gaucher disease is due to mutations involving the glucocerebrosidase gene. A closely homologous pseudogene is located approximately 16 kD downstream from the functional gene. Sequence analysis of clones from cDNA libraries made from skin fibroblast cultures showed several independent clones with the sequence of an aberrantly processed pseudogene message. Examination of cellular RNA from lymphob...
نمودار تعداد نتایج جستجو در هر سال
با کلیک روی نمودار نتایج را به سال انتشار فیلتر کنید