I-SceI is a homing endonuclease that specifically cleaves an 18-bp double-stranded DNA. I-SceI exhibits a strong preference for cleaving the bottom strand DNA. The published structure of I-SceI bound to an uncleaved DNA substrate provided a mechanism for bottom strand cleavage but not for top strand cleavage. To more fully elucidate the I-SceI catalytic mechanism, we determined the X-ray struct...

Donepezil, the most widely used drug for treatment of Alzheimer's disease (AD), is an acetylcholinesterase (AChE) inhibitor and thought to improve cognition by stimulating cholinergic neurotransmission. However, no correlation has yet been established between inhibitory role AChE inhibitors their therapeutic effects when in AD patients. The cleavage pathway amyloid precursor protein (APP) inclu...

Exposure to ionizing radiation (IR) during procedures such as abdominal and pelvic radiotherapy results in severe intestinal injury. In the current study, we demonstrated that a lethal dose of IR triggered epithelial cell apoptosis pyroptosis via Caspase-3 cleavage. The ginseng active ingredients, ginsenosides (GS) phenolic acids (GPA), significantly inhibited IR-induced IEC-6 cells. GS GPA als...

RhoA controls cleavage furrow formation during cell division, but whether RhoA suffices to orchestrate spatiotemporal dynamics of furrow formation is unknown. In this issue, Wagner and Goltzer (2016. J. Cell Biol http://dx.doi.org/10.1083/jcb.201603025) show that RhoA activity can induce furrow formation in all cell cortex positions and cell cycle phases.

Due to its artificial and unnatural structure, Israeli society has since the beginning of its establishment, been a context for the emergence of different kinds of cleavages and tensions. This state of affairs has endangered the political stability of the Israeli regime. The present research is trying to answer this question: what is the effect of religious-secular cleavages in Israel on the fu...

Background: It was reported in a literature that approximately one third of the 4-cell stage embryos did not exhibit a tetrahedral shape. Non-tetrahedral embryos showed a lower in vitro developmental potential than tetrahedral embryos. Recently vitrification technology has been widely employed for embryo cryopreservation. The objective of this study was to prove our hypothesis that vitrified - ...

The question of how the site for division of the cytoplasm is determined at the end of mitosis has been studied for over a century, and it remains an active, controversial and fascinating problem in cell biology. This problem draws on the use of several model cell types, with the goal of understanding and identifying how the cell cycle regulates signals between the mitotic apparatus and the cel...