The goal of this study was to assess the effect of multi-dose St Thomas' cardioplegia on intracellular sodium homeostasis in a rat heart model. A new magnetic resonance method was applied which enable us to detect intracellular Na changes without chemical shift reagents. Three groups of isolated rat hearts were subjected to 51 min of ischemia and 51 min of reperfusion at 37 degrees C: Group 1-t...

OBJECTIVE: Myocardial protection is the most important in cardiac surgery. We compared our modified single-dose long-acting lignocaine-based blood cardioplegia with short-acting St Thomas 1 blood cardioplegia in patients undergoing single valve replacement. METHODS: A total of 110 patients who underwent single (aortic or mitral) valve replacement surgery were enrolled. Patients were divided i...

Objectives: The main objective of the study is to find comparison CKMB, Lactate levels and ejection fraction with warm cold cardioplegia on first post OP day in elective CABG cases. Material methods: This cross sectional was conducted Armed forces institute cardiology AFIC /NIHD Rawalpindi during 1st October 2022 30th November 2022. Data collected permission ethical committee hospital. variable...

BACKGROUND The advantages of blood cardioplegia include the oxygen-carrying capacity, superior oncotic and buffering properties, and endogenous antioxidants contained in blood. However, the partial dilution of blood in 4:1 (blood:crystalloid) cardioplegic solutions may nullify these advantages and progressively dilute blood during continuous retrograde delivery. This study tested the hypothesis...

In most adult cardiac surgery operations, the heart must be completely immobile and isolated from blood. Therefore, is stopped in diastole a still operative site obtained. Cardiac arrest results ischemia-reperfusion injury. For these reasons, myocardial protection prevention of damages are required. Various cardioplegia solutions used for this purpose. It can said that gold standard method arre...

Cardioplegia is used to partially alleviate the effects of surgically induced global ischemia injury; however, the molecular mechanisms involved in this cardioprotection remain to be elucidated. To improve the understanding of the molecular processes modulating the effects of global ischemia and the cardioprotection afforded by cardioplegia, we constructed rabbit heart cDNA libraries and isolat...

it has been known that cardiomyocytes possess a remarkable ability to downregulate their energy expenditure on restricted 02 supply. however it has also been speculated that an ability to suppress aerobic respiration and atp utilization could be a protective response to prolonged hypoxia. having the role of the cytochrome oxidase enzyme in mind, one can ask if its inhibition by using cyanide ha...

Objective: Adenoviral gene transfer to the arrested heart during cardiopulmonary bypass (CPB) is a novel method of allowing prolonged vector contact with the myocardium. In this model we investigated the importance of temperature, duration of arrest and cardioplegia on transgene expression. Methods: First-generation adenoviral vector (1 £ 10 total viral particles) containing the transgene for t...

BACKGROUND NO has been advocated as an adjunct to cardioplegia solutions. However, NO undergoes a rapid biradical reaction with superoxide anions to produce peroxynitrite (ONOO(-)). ONOO(-) in crystalloid cardioplegia solution induces injury to coronary endothelium and to systolic function after cardioplegia and reperfusion. However, ONOO(-) may be degraded to less lethal or cardioprotective in...