Recently, a new approach of upstream detection of BVD virus in raw, unprocessed FBS has been described. This is a sandwich enzyme-linked immunosorbent assay (S-ELISA) designed to detect presence of BVD virus in the serum, using a commercially available diagnostic kit (Syracuse Bioanalytical, Syracuse, NY). In this study, the S-ELISA kit that uses raw (unprocessed) FBS as the testing sample was ...

BACKGROUND Autologous vein grafts and balloon angioplasty are still commonly used for arterial reconstructive procedures. Their success is limited by the development of intimal hyperplasia (IH). Biliverdin (BVD), one of the by-products of heme degradation, has been shown to have potent antioxidant and antiinflammatory effects. We hypothesized that BVD administration would protect vascular tissu...

Submit Manuscript | http://medcraveonline.com Abbreviations: MCWF: Mycobacterium Cell Wall Fraction; BRDC: Bovine Respiratory Disease Complex; ADG: Average Daily Gain; IL-2:Interleukin-2; IL-6: Interleukin-6; IFN-γ: Interferon Gamma; TDM: Trehalose 6,6′-Dimycolate; MDP: Muramyl Dipeptide; IBR: Infectious Bovine Rhinotracheitis; PI3: Parainfluenza 3; BRSV: Bovine Respiratory Syncytial virus; BVD...

During a study on Bovine Viral Diarrhoea (BVD) epidemiology in Thailand, a pestivirus was detected in serum from a calf. Comparative nucleotide sequence analysis showed that this virus was closely related to a recently described atypical pestivirus (D32/00_'HoBi') that was first isolated from a batch of foetal calf serum collected in Brazil. The results from virus neutralisation tests performed...

BACKGROUND Bovine viral diarrhea (BVD) is an economically important disease of cattle distributed worldwide. Diagnosis of BVD relies on laboratory-based detection of its viral causing agent or virus specific antibodies and the most common laboratory method for this purpose is Enzyme-Linked Immunosorbent Assay (ELISA). OBJECTIVES The current study was aimed to develop a simple indirect ELISA t...

Quantitative real-time RT-PCR (qRT-PCR) assay was developed for the detection and quantification of bovine viral diarrhea virus (BVDV) in clinical samples from persistently infected cattle. qRT-PCR was optimized to quantify the number of BVD virus copies using Light Cycler® detection system and intercalation fluorogenic dye SYBR Green I. A universal set of primers was selected from a highly con...

conclusions the newly developed simple indirect elisa showed high sensitivity and specificity with respect to vnt. developing such a simple, sensitive, and specific elisa which is much less expensive than the available commercial elisa kits can improve the detection of bvdv infections, help to eliminate the disease from herds, and decrease economic losses caused by this disease. background bovi...

Bovine viral diarrhoea virus (BVDV) is an economically important pathogen of cattle worldwide. Infection of a pregnant animal may lead to persistent infection of the foetus and birth of a persistently infected (PI) calf that sheds the virus throughout its life. However, BVD viruses are not strictly species specific. BVDV has been isolated from many domesticated and wild ruminants. This is of pr...

Bovine cell cultures infected with bovine viral diarrhoea-mucosal disease (BVD) virus were radiolabelled with L-[35S]methionine or D-[2-3H]mannose followed by analysis of the labelled polypeptides by radioimmunoprecipitation and polyacrylamide gel electrophoresis in one and two dimensions. Six glycoproteins were detected in infected cells. Two abundant species had Mr of 48K and 56K to 58K while...

The Boogie Verification Debugger (BVD) is a tool that lets users explore the potential program errors reported by a deductive program verifier. The user interface is like that of a dynamic debugger, but the debugging happens statically without executing the program. BVD integrates with the programverification engine Boogie. Just as Boogie supports multiple language front-ends, BVD can work with...