Bioluminescence represents an operational threat to naval nighttime operations because the flow field associated with their motion stimulates naturally occurring plankton. In the littoral, the primary sources of bioluminescence are dinoflagellates, common unicellular plankton that are also known to form red tides. Dinoflagellate bioluminescence is stimulated by flow stress of sufficient magnitu...

BACKGROUND Most methods for monitoring glucose level require an external energy source which may limit their application, particularly in vivo test. Bioluminescence technique offers an alternative way to provide emission light without external energy source by using bioluminescent proteins found from firefly or marine vertebrates and invertebrates. For quick and non-invasive detection of glucos...

We generated a mouse model (MIP-Luc-VU-NOD) that enables non-invasive bioluminescence imaging (BLI) of beta cell loss during the progression of autoimmune diabetes and determined the relationship between BLI and disease progression. MIP-Luc-VU-NOD mice displayed insulitis and a decline in bioluminescence with age which correlated with beta cell mass, plasma insulin, and pancreatic insulin conte...

BACKGROUND The porcine oocyte maturation in vivo occurs within the ovarian follicle and is regulated by the interactions between oocytes and surrounding follicular components, including theca, granulosa, and cumulus cells, and follicular fluid. Therefore, the antral follicle is an essential microenvironment for efficient oocyte maturation and its developmental competence. Quantitative biolumine...

photoproteins are excellent reporter systems because they don’t have virtually background signal. aequorin is the most well-known photoprotein. three improved engineered photoproteins photina, i-photina and c-photina, were also recently developed and optimized for generation of ca2+ mobilization assays precisely. the total light emission is greater than aequorin and their reaction kinetics is a...

The glowworm Arachnocampa flava is a carnivorous fly larva (Diptera) that uses light to attract prey into its web. The light organ is derived from cells of the Malpighian tubules, representing a bioluminescence system that is unique to the genus. Bioluminescence is modulated through the night although light levels change quite slowly compared with the flashing of the better-known fireflies (Col...

A Lactococcus lactis subsp. lactis strain that can sense the bacteriocin nisin and transduce the signal into bioluminescence was constructed. By using this strain, a bioassay based on bioluminescence was developed for quantification of nisin, for detection of nisin in milk, and for identification of nisin-producing strains. As little as 0.0125 ng of nisin per ml was detected within 3 h by this ...