نتایج جستجو برای: and ns5a proteins

تعداد نتایج: 16874701  

2014
James J Kohler James H Nettles Franck Amblard Selwyn J Hurwitz Leda Bassit Richard A Stanton Maryam Ehteshami Raymond F Schinazi

Recent progress in the understanding of hepatitis C virus (HCV) biology and the availability of in vitro models to study its replication have facilitated the development of direct-acting antiviral agents (DAAs) that target specific steps in the viral replication cycle. Currently, there are three major classes of DAA in clinical development: NS3/4A protease inhibitors, NS5B polymerase inhibitors...

Journal: :PLoS Pathogens 2008
Nicole Appel Margarita Zayas Sven Miller Jacomine Krijnse-Locker Torsten Schaller Peter Friebe Stephanie Kallis Ulrike Engel Ralf Bartenschlager

Persistent infection with the hepatitis C virus (HCV) is a major risk factor for the development of liver cirrhosis and hepatocellular carcinoma. With an estimated about 3% of the world population infected with this virus, the lack of a prophylactic vaccine and a selective therapy, chronic hepatitis C currently is a main indication for liver transplantation. The establishment of cell-based repl...

Journal: :Journal of virology 2008
Toru Okamoto Hiroko Omori Yuuki Kaname Takayuki Abe Yorihiro Nishimura Tetsuro Suzuki Tatsuo Miyamura Tamotsu Yoshimori Kohji Moriishi Yoshiharu Matsuura

Hepatitis C virus (HCV) nonstructural protein 5A (NS5A) regulates viral replication through its interaction with host and other viral proteins. We have previously shown that FK506-binding protein 8 (FKBP8) binds to NS5A and recruits Hsp90 to form a complex that participates in the replication of HCV. In this study, we examined the biochemical characteristics of the interaction and the intracell...

Journal: :Scientific reports 2016
Gavin Ka Yu Siu Fan Zhou Mei Kuen Yu Leiliang Zhang Tuanlao Wang Yongheng Liang Yangchao Chen Hsiao Chang Chan Sidney Yu

Hepatitis C virus (HCV) has long been observed to take advantage of the host mitochondria to support viral replication and assembly. The HCV core protein has been implicated to fragment host mitochondria. In this report, we have discovered that the non-structural protein 5A (NS5A) plays an instructive role in attaching ER with mitochondria, causing mitochondrial fragmentation. Dynamin-related p...

Journal: :Journal of virology 2011
Kristi L Berger Sean M Kelly Tristan X Jordan Michael A Tartell Glenn Randall

Phosphatidylinositol 4-kinase III alpha (PI4KA) is an essential cofactor of hepatitis C virus (HCV) replication. We initiated this study to determine whether HCV directly engages PI4KA to establish its replication. PI4KA kinase activity was found to be absolutely required for HCV replication using a small interfering RNA transcomplementation assay. Moreover, HCV infection or subgenomic HCV repl...

Journal: :Journal of virology 2011
Robert A Fridell Dike Qiu Lourdes Valera Chunfu Wang Ronald E Rose Min Gao

BMS-790052, targeting nonstructural protein 5A (NS5A), is the most potent hepatitis C virus (HCV) inhibitor described to date. It is highly effective against genotype 1 replicons and also displays robust genotype 1 anti-HCV activity in the clinic (M. Gao et al., Nature 465:96-100, 2010). BMS-790052 inhibits genotype 2a JFH1 replicon cells and cell culture infectious virus with 50% effective con...

Journal: :The Journal of general virology 2008
Jamel Mankouri Andrew Milward Kenneth R Pryde Lucile Warter Annette Martin Mark Harris

GB virus B (GBV-B) is the closest relative to hepatitis C virus (HCV) with which it shares a common genome organization, however, unlike HCV in humans, it generally causes an acute resolving hepatitis in New World monkeys. It is important to understand the factors regulating the different disease profiles of the two viruses and in this regard, as well as playing a key role in viral RNA replicat...

Journal: :Gut 2004
O Núñez A Fernández-Martínez P L Majano A Apolinario M Gómez-Gonzalo I Benedicto M López-Cabrera L Boscá G Clemente C García-Monzón P Martín-Sanz

BACKGROUND Cyclooxygenase 2 (COX-2) and matrix metalloproteinases (MMPs) have been implicated in tissue injury and fibrogenesis in animal models but little is known regarding their role in hepatitis C virus (HCV) related liver disease in humans. AIMS To characterise the intrahepatic expression pattern of COX-2 and MMPs in chronic HCV infection and determine whether HCV core and NS5A proteins ...

2017
Tiffany Benzine Ryan Brandt William C Lovell Daisuke Yamane Petra Neddermann Raffaele De Francesco Stanley M Lemon Alan S Perelson Ruian Ke David R McGivern

Hepatitis C virus (HCV) RNA is synthesized by the replicase complex (RC), a macromolecular assembly composed of viral non-structural proteins and cellular co-factors. Inhibitors of the HCV NS5A protein block formation of new RCs but do not affect RNA synthesis by pre-formed RCs. Without new RC formation, existing RCs turn over and are eventually lost from the cell. We aimed to use NS5A inhibito...

Journal: :Hepatology 2010
Wagane J A Benga Sophie E Krieger Maria Dimitrova Mirjam B Zeisel Marie Parnot Joachim Lupberger Eberhard Hildt Guangxiang Luo John McLauchlan Thomas F Baumert Catherine Schuster

UNLABELLED Chronic hepatitis C virus (HCV) infection is a major cause of liver disease worldwide. Restriction of HCV infection to human hepatocytes suggests that liver-specific host factors play a role in the viral life cycle. Using a yeast-two-hybrid system, we identified apolipoprotein E (apoE) as a liver-derived host factor specifically interacting with HCV nonstructural protein 5A (NS5A) bu...

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