Synthesis of a polysaccharide adhesin by icaADBC-encoded enzymes is currently the best-understood mechanism of staphylococcal biofilm development. In four methicillin-resistant Staphylococcus aureus isolates, environmental activation of icaADBC did not always correlate with increased biofilm production. Moreover, glucose-mediated biofilm development in these isolates was icaADBC independent. Ap...

objectives we investigated the potential relationships between biofilm formation and prevalence of virulence genes (asa1, esp, cyla, and gele/spre), and antimicrobial resistance genes (aac (6’)/aph (2”) in enterococcus faecalis isolated from patients with urinary tract infection. patients and methods in this survey 95 e. faecalis isolates from patients with urinary tract infections staying at s...

Pseudomonas aeruginosa is a clinically relevant species involved in biofilm-based chronic infections. We provide evidence that the P. aeruginosa LapG protein functions as a periplasmic protease that can cleave the protein adhesin CdrA off the cell surface, and thereby plays a role in biofilm formation and biofilm dispersal. The P. aeruginosa LapG protein is shown to be a functional homolog of t...

coastal region of chah bahar port, sea of oman, was screened for the presence of bioluminescence bacteria for the first time. water samples were taken from surface and subsurface layers and immediately spread on nutrient seawater complete (swc) agar. luminous colonies were observed after an overnight incubation at 25°c. among twenty luminous isolates, four of them were selected for preliminary ...

Background & Objectives:  Methicillin resistance Staphylococcus aureus (MRSA) is one of the most important pathogens that causes several nosocomial and community infections. Adhesion to surfaces and biofilm formation is considered main step in staphylococcal infection. The aims of this study were to determine presence oficaABCD genes and relation to the biofilm formation in of...

The impact of many microorganisms on their environment depends upon their ability to form surface bound communities called biofilms [1]. Biofilm formation on implanted medical devices has severe consequences for human health by providing both a portal of entry and a sanctuary for invasive bacterial and fungal pathogens [1 and 2]. Biofilm regulators and adherence molecules are extensively define...

Bacterial biofilms represent a promising opportunity for engineering of microbial communities. However, our ability to control spatial structure in biofilms remains limited. Here we engineer Escherichia coli with a light-activated transcriptional promoter (pDawn) to optically regulate expression of an adhesin gene (Ag43). When illuminated with patterned blue light, long-term viable biofilms wit...

The pathogenesis of Staphylococcus epidermidis is correlated with biofilm formation. We investigated the effect of three common alcoholic skin disinfectants, ethanol, n-propanol and isopropanol, on the biofilm formation of 37 clinical, icaADBC-positive S. epidermidis isolates. In alcohol-supplemented media 18 strains displayed increased biofilm expression. Sixteen of 19 strains were generally i...

background: the aim of this study was to investigate the antibiotic susceptibility pattern as well as the phenotypic and genotypic biofilm formation ability of staphylococcus aureus isolates from patients with urinary tract infection (uti). methods: a total of 39 isolates of s. aureus were collected from patients with uti. the antibiotic susceptibility patterns of the isolates were determined b...