A method was devised for infecting Anthurium andraeanum Linden ex André, an economically important ornamental monocot, with Agrobacterium tumefaciens. Tumors were obtained on plant stems 7 to 10 weeks after inoculation with oncogenic A. tumefaciens strains C58 and A281 cultured previously in an induction medium containing 200 μM acetosyringone at pH 5.5. A higher percentage of tumors were forme...

Inducible T-strand mobilization from the Ti plasmid of Agrobacterium tumefaciens to the genome of a plant host is mediated by the activation of a cascade of bacterial virulence genes. It is initiated when the bacterium senses the presence of a low molecular weight inducer secreted by the plant. Although many hydroxyphenylpropanoid and phenolic compounds can activate the virulence cascade, the o...

………………………………………………………………………………….…...vii Chapter 1. Introduction .…………………………………………………………….……….....1 Chapter 2. Review of Literature ...…………………………………………………….………. 3 2.1 Molecular Mechanism of Agrobacterium-mediated DNA Transfer ……...........…3 2.2 Adoption for Plant Molecular Biology ………….……………………………...10 2.3 Agrobacterium-mediated Plant Transformation Protocol Development ….…...12 Chapter 3. Mate...

The biobleachability of soda-anthraquinone pulp from orange tree pruning has been evaluated in this study for the first time. Three different laccase-mediator systems (LMS) have been tested: laccase from Trametes villosa (Tv), either in combination with 1-hydroxybenzotriazole (HBT) or with acetosyringone (AS), and laccase from Myceliophthora thermophila (Mt) in combination with AS. After the LM...

A virB-lacZ translational fusion was constructed to monitor expression of the Agrobacterium tumefaciens virB operon. Expression of the fusion gene was dependent on the presence of pTiA6 virA, virG, and a plant factor acetosyringone. Analysis of deletion mutants, constructed by exonuclease Bal31 digestion, showed that 68 residues upstream of the virB transcription initiation site was necessary f...