This dissertation is devoted to better understanding the role that fluids play in the selection of the shapes and functions of objects and creatures in nature. Toward that end, three specific examples are considered: stalactites, icicles, and species of colonial green algae known as Volvox. In the cases of stalactites and icicles, the object's growth is considered as a free-boundary problem. Fo...

We previously showed that blind rats whose vision was restored by gene transfer of Chlamydomonas channelrhodopsin-2 (ChR2) could only detect wavelengths less than 540 nm because of the action spectrum of the transgene product. Volvox-derived channelrhodopsin-1, VChR1, has a broader spectrum than ChR2. However, the VChR1 protein was mainly localized in the cytoplasm and showed weak ion channel p...

BACKGROUND The multicellular green alga Volvox carteri represents an attractive model system to study various aspects of multicellularity like cellular differentiation, morphogenesis, epithelial folding and ECM biogenesis. However, functional and molecular analyses of such processes require a wide array of molecular tools for genetic engineering. So far there are only a limited number of molecu...

Volvox and its relatives provide an exceptional model for integrative studies of the evolution of multicellularity and cellular differentiation. The volvocine algae range in complexity from unicellular Chlamydomonas through several colonial genera with a single cell type, to multicellular Volvox with its germ-soma division of labor. Within the monophyletic family Volvocaceae, several species of...

Flagella-generated fluid stirring has been suggested to enhance nutrient uptake for sufficiently large micro-organisms, and to have played a role in evolutionary transitions to multicellularity. A corollary to this predicted size-dependent benefit is a propensity for phenotypic plasticity in the flow-generating mechanism to appear in large species under nutrient deprivation. We examined four sp...

Volvox is a very interesting oogamous organism that exhibits various types of sexuality and/or sexual spheroids depending upon species or strains. However, molecular bases of such sexual reproduction characteristics have not been studied in this genus. In the model species V. carteri, an ortholog of the minus mating type-determining or minus dominance gene (MID) of isogamous Chlamydomonas reinh...

Volvox is a multicellular spherical green alga that is closely related to the more widely studied unicellular Chlamydomonas. Volvox is a fascinating creature that serves as a useful model system for investigating the evolution of multicellularity and the regulation of cell differentiation (reviewed in Kirk and Nishii, 2001). An adult Volvox, called a spheroid, consists of several thousand small...