نتایج جستجو برای: voltage clamp

تعداد نتایج: 123628  

Journal: :Journal of neurophysiology 2009
Jorge Golowasch Gladis Thomas Adam L Taylor Arif Patel Arlene Pineda Christopher Khalil Farzan Nadim

During growth or degeneration neuronal surface area can change dramatically. Measurements of membrane protein concentration, as in ion channel or ionic conductance density, are often normalized by membrane capacitance, which is proportional to the surface area, to express changes independently from cell surface variations. Several electrophysiological protocols are used to measure cell capacita...

Journal: :Journal of General Physiology 1969

Journal: :Biophysical journal 2003
Andreas T Schaefer Moritz Helmstaedter Bert Sakmann Alon Korngreen

To understand functions of a single neuron, such as propagation and generation of synaptic or action potentials, a detailed description of the kinetics and distribution of the underlying ionic conductances is essential. In voltage-clamp experiments, incomplete space clamp distorts the recorded currents, rendering accurate analysis impossible. Here, we present a simple numerical algorithm that c...

Journal: :Journal of General Physiology 1965

Journal: :Neuroscience letters 1993
M Juusola M Weckström

The membrane properties of short type blowfly photoreceptors (R1-6) were investigated in dark and light adaptation with single electrode current and voltage clamp techniques. The impedance of the cells was defined in frequency domain by using discontinuous current clamp and white-noise-modulated current injection. We found that the slow activation and relaxation of the voltage-dependent K+ cond...

Journal: :Journal of neurophysiology 2015
Reid R Harrison Ilya Kolb Suhasa B Kodandaramaiah Alexander A Chubykin Aimei Yang Mark F Bear Edward S Boyden Craig R Forest

Patch clamping is a gold-standard electrophysiology technique that has the temporal resolution and signal-to-noise ratio capable of reporting single ion channel currents, as well as electrical activity of excitable single cells. Despite its usefulness and decades of development, the amplifiers required for patch clamping are expensive and bulky. This has limited the scalability and throughput o...

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