Supernatant fractions obtained by high-speed centrifugation of homogenates of liver and chemically induced primary and transplantable hepatomas were assayed kinetically, and by starch gel electrophoresis and column chromatography on di ethylaminoethyl (DEAE)-cellulose, for isoenzymes of glucose ATP phosphotransferase activity. Well and highly differenti ated hepatomas were similar to liver in h...

THIRTY years ago Takeo Maruyama, Ranajit Chakradominant selection can be much greater than that mainborty, and I published an article about the effects tained by mutation-selection balance, but that overdomof bottlenecks on genetic variation (Nei et al. 1975). This inant selection incurs a large genetic load (genetic death article was motivated by two controversies occurring in or fertility exc...

Immunoprecipitations of hepatic lipase from pulselabeled rat liver have demonstrated that hepatic lipase is synthesized in two distinct molecular weight forms, HL-I (M, = 51,000) and HL-I1 (M, = 53,000). Both forms are immunologically related to purified hepatic lipase, but not to lipoprotein lipase. HL-I and HL-11 are also kinetically related and represent different stages of intracellular pro...

Cereal Chem. 72(6):527-532 Wheat protein fractions, separated by the Osborne Solvent Fractionaseparated by HPCE. Combined results of this study and our previous tion Procedure, were characterized by high-performance capillary elecstudies provide a catalog of individual gliadin information from HPCE, trophoresis (HPCE). Each fraction was separated on a 27-cm fused-glass HPLC, acid-polyacrylamide...

LANDI, S. (University of Toronto, Toronto, Ontario, Canada), AND H. R. HELD. Physicochemical and biological studies on various preparations of tuberculin purified protein derivative. Appl. Microbiol. 13:132-139. 1965.-Tuberculin purified protein derivative (PPD) has been prepared by seven different precipitation methods from culture filtrate of Mycobacterium tuberculosis var. hominis. It was fo...

The mechanism of action of T4-induced polynu-cleotide kinase on short single-stranded deoxyoligonucleotideshas been investigated.It was found that the phosphorylationreaction catalyzed by T, polynucleotide kinase could be re-versed. Thus, a 5 ’-3*P-labeleddeoxyoligonucleotide and ADPon incubation with the enzyme formed [Y-~~PJATP.In addi-tion some radioac...

The 0AL[0RS wheatÐrye chromosomal translocation originally found in {Amigo| wheat possesses resistance genes for stem rust\ powdery mildew and greenbug biotypes B and C\ but also has a negative e}ect on wheat processing quality[ Recently\ a second 0AL[0RS translocation carrying Gb5\ a gene conferring resistance to greenbug biotypes B\ C\ E\ G and I\ was identi_ed in the wheat germplasm line {GR...

Tumor cells undergo self-destruction when incubated with cytotoxic T-cells (CTL) consistent with the observation that suppression of target protein synthesis causes resistance to apoptosis. Resistance to CTL is also induced by stress, suggesting that pathways exist suppressing apoptosis. Here we examine whether stress induced lysis resistance to CTL and tumor necrosis factor a involves stress p...

A Piscirickettsia salmonis was isolated from the brain of a diseased coho salmon during an outbreak in Southern Chile. The isolate, AS-98, showed similar genetic, biochemical, serological and virulence characteristics to the type strain LF-89. The brain might represent an important residence site for P.salmonis. Piscirickettsia salmonis is an endemic and widely distributed pathogen among cultur...