It was a strange choice to ask me, who has not contributed to the description of the titration behavior of proteins, to discuss tha t subject. Nevertheless I am pleased to attempt to summarize the state of knowledge for our present purposes. Our best objective in this monograph is surely to make ourselves and each other better informed; we will be examining the meaning of terms for that purpose...

A new measuring method is described for obtaining a proton titration curve. The curve is obtained from a microporous composite membrane, consisting of polystyrene beads in an agarose matrix, with lysozyme molecules adsorbed to the bead surface. The membrane is incorporated into a sensor system by deposition on a silicon chip with a pH-sensitive ion-sensitive field effect transistor (ISFET) loca...

Atomic force microscopy was used to measure the forces between a silica probe and a carboxylic acidterminated self-assembledmonolayer (SAM) onagold substrate in the presence ofKCl electrolyte solutions of different pH. Silica-silica interaction force measurements were conducted at different pH values to determine the silica probe surface electrostatic potentials under these conditions. The inte...

1. Carbon dioxide titration curves were determined in vivo in dog and man at various degrees of acute non-respiratory acidaemia and alkalaemia. 2. The slope of the CO2 titration curve (delta log Pco2/delta pH) was found to increase with the severity of the acute non-respiratory alkalaemia the slope (delta log Pco2/delta pH) tended towards unity. 3. A simple scheme based on the CO2 titration cur...

Titration of N- and B-tropic murine leukemia viruses on sensitive and resistant cell lines has been studied by direct XC plaque assay and infective center assay. The titration of cloned B-tropic virus by infective center assay on BALB/3T3 (Fv-1b/b) and NIH/3T3 (Fv-1n/n) cells gave one-hit patterns, with 100-fold less infected NIH/3T3 cells than BALB/3T3 cells. The titration of B-tropic virus on...

The study of 14 normal young men by glucose titration procedures has defined the magnitude of splay in this population, differing from previously reported data in its unexpected deviation from the line of theoretic unity high on the titration curve. Compared to these normal subjects, a group of glucosuric men could be divided into two subclasses, those with normal maximal rate of glucose reabso...

The histidine C-2 proton NMR titration curves of ribonuclease S-peptide (residues 1 to 20) and S-protein (residues 21 to 124) are reported. Although S-protein contains 3 histidine residues, four discrete resonances are observed to titrate. One of these arises from the equivalent histidine residues of unfolded S-protein. The variation in area of the four resonances indicate that there is a rever...

The effects of sodium n-dodecyl sulphate (SDS) on the structure of histone HI has been studied by a combination of e:quilibrium dialysis, U.V. spectroscopy ; polyacrylamide gel electrophoresis, protein titration and viscometery techniques using, 2.5 mM phosphate buffer, pH 6.4. The interaction of H, and SDS in contrast tomanyothel-protein-SDS interactions is organized between V 40 to 70. A...