BACKGROUND The European DRUID (Driving under the Influence of Drugs, Alcohol And Medicines) project calls for analysis of oral fluid (OF) samples, collected randomly and anonymously at the roadside from drivers in Denmark throughout 2008-2009. To analyze these samples we developed an ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method for detection of 29 drugs a...

BACKGROUND Given that Δ(9)-tetrahydrocannabinol, the active constituent of cannabis, has been shown to greatly reduce driving ability, thus being linked to many drug driving accidents, its reliable detection is of great importance. RESULTS An optimised carbon paste electrode, fabricated from graphite powder and mineral oil, is utilised for the sensitive detection of Δ(9)-tetrahydrocannabinol ...

The detection and quantitation of drugs of abuse in blood is of growing interest in forensic and clinical toxicology. With the development of highly sensitive chromatographic methods, such as high-performance liquid chromatography (HPLC) with sensitive detectors and gas chromatography-mass spectrometry (GC-MS), more and more substances can be determined in blood. This review includes methods fo...

An analytical procedure for the determination of Delta9-tetrahydrocannabinol (THC), 11-nor-9-carboxy-Delta9-tetrahydrocannabinol (THCA), and 11-hydroxy-Delta9-tetrahydrocannabinol (11-OH-THC) in whole blood has been developed and validated using liquid chromatography with tandem mass spectral detection (MS). Cannabinoids present in the blood samples were quantified using solid-phase extraction ...

An ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS-MS) method was developed and validated for the simultaneous determination of Delta(9)-tetrahydrocannabinol (THC), 11-hydroxy-Delta(9)-tetrahydrocannabinol (11-OH-THC), and 11-nor-9-carboxy-Delta(9)-tetrahydrocannabinol (THC-COOH) in whole blood. Samples were prepared by protein precipitation followed by solid-phase ext...

The aim of this study was to determine if chronic, low-dose administration of a nonspecific cannabinoid receptor agonist could provide cardioprotective effects in a model of type I diabetes mellitus. Diabetes was induced in eight-week-old male Wistar-Kyoto rats via a single intravenous dose of streptozotocin (65 mg kg-1). Following the induction of diabetes, Δ9-tetrahydrocannabinol was administ...

BackgroundTourette syndrome is characterized by chronic motor and vocal tics. There preliminary evidence of benefit from cannabis products containing Δ9-tetrahydrocannabinol (THC) that coadministration cannabidiol (CBD) improves the side-effect profile safety.MethodsIn this double-blind, crossover trial, participants with severe Tourette were randomly assigned to a 6-week treatment period escal...