نتایج جستجو برای: subcutaneous human preadipocyte

تعداد نتایج: 1671344  

Journal: :PloS one 2016
Xiaoling Chen Bo Zhou Yanliu Luo Zhiqing Huang Gang Jia Guangmang Liu Hua Zhao

The fat mass and obesity associated (FTO) gene plays an important role in adipogenesis. However, its function during porcine intramuscular preadipocyte proliferation and differentiation remains poorly understood. In this study, we prepared the antiserum against porcine FTO (pFTO), which was used to determine its subcellular localization and tissue distribution. Our data indicated that pFTO was ...

2006
Tamara Tchkonia Marc Lenburg Thomas Thomou Nino Giorgadze Garrett Frampton Tamar Pirtskhalava Andrew Cartwright Mark Cartwright John Flanagan Iordanes Karagiannides Norman Gerry R. Armour Forse Yourka Tchoukalova Michael D. Jensen Charalabos Pothoulakis James L. Kirkland

Anatomically separate fat depots differ in size, function, and contribution to pathological states such as the metabolic syndrome. We isolated preadipocytes from different human fat depots to determine if the basis for this variation is partly attributable to differences in inherent properties of fat cell progenitors. We found that genome-wide expression profiles of primary preadipocytes cultur...

2010
Odile Poulain-Godefroy Olivier Le Bacquer Pauline Plancq Cécile Lecœur François Pattou Gema Frühbeck Philippe Froguel

It was recently demonstrated that TLR4 activation via dietary lipids triggers inflammatory pathway and alters insulin responsiveness in the fat tissue during obesity. Here, we question whether other TLR family members could participate in the TLR-mediated inflammatory processes occurring in the obese adipose tissue. We thus studied the expression of TLR1, TLR2, TLR4, and TLR6 in adipose tissue....

Journal: :American journal of physiology. Endocrinology and metabolism 1998
M McIntosh D Hausman R Martin G Hausman

The purpose of this study was to determine whether the antiobesity actions of dehydroepiandrosterone (DHEA) are due to an influence on preadipocyte proliferation and/or differentiation in primary cultures of pig and rat stromal-vascular (SV) cells. Pig SV cells were isolated from dorsal subcutaneous adipose tissue of 7-day-old pigs. For the proliferation assays, pig SV cells were grown for 4 da...

2009
Petter Isakson Ann Hammarstedt Birgit Gustafson Ulf Smith

OBJECTIVE We examined preadipocyte differentiation in obese and nonobese individuals and the effect of cytokines and wingless-type MMTV (mouse mammary tumor virus) integration site family, member 3A (Wnt3a) protein on preadipocyte differentiation and phenotype. RESEARCH DESIGN AND METHODS Abdominal subcutaneous adipose tissue biopsies were obtained from a total of 51 donors with varying BMI. ...

Journal: :Biochemical and biophysical research communications 2009
Takeo Iwata Masamichi Kuwajima Akiko Sukeno Naozumi Ishimaru Yoshio Hayashi Martin Wabitsch Noriko Mizusawa Mitsuo Itakura Katsuhiko Yoshimoto

Obesity is considered a chronic low-grade inflammatory status and the stromal vascular fraction (SVF) cells of adipose tissue (AT) are considered a source of inflammation-related molecules. We identified YKL-40 as a major protein secreted from SVF cells in human visceral AT. YKL-40 expression levels in SVF cells from visceral AT were higher than in those from subcutaneous AT. Immunofluorescence...

Journal: :Endocrinology 2006
Soraya Taleb Raffaella Cancello Karine Clément Daniele Lacasa

We previously showed that the cysteine protease cathepsin S (CTSS), known to degrade several components of the extracellular matrix (ECM), is produced by human adipose cells and increased in obesity. Because ECM remodeling is a key process associated with adipogenesis, this prompted us to assess the potential role of CTSS to promote preadipocyte differentiation. Kinetic studies in primary human...

Journal: :Blood 2014
Vamsee D Myneni Kiyotaka Hitomi Mari T Kaartinen

Factor XIII-A (FXIII-A) transglutaminase (TG) was recently identified as a potential causative obesity gene in human white adipose tissue (WAT). Here, we have examined the role of TG activity and the role of protein crosslinking in adipogenesis. Mouse WAT and preadipocytes showed abundant TG activity arising from FXIII-A. FXIII-A was localized to the cell surface and acted as a negative regulat...

2011
José María Moreno-Navarrete Francisco J. Ortega José-Ignacio Rodríguez-Hermosa Mònica Sabater Gerard Pardo Wifredo Ricart José Manuel Fernández-Real

OBJECTIVE Metformin has been well characterized in vitro as a substrate of liver-expressed organic cation transporters (OCTs). We investigated the gene expression and protein levels of OCT-1 and OCT-2 in adipose tissue and during adipogenesis and evaluated their possible role in metformin action on adipocytes. RESEARCH DESIGN AND METHODS OCT1 and OCT2 gene expressions were analyzed in 118 adi...

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