This work aimed at determining the ideal ischemia time in an in vitro ischemia-reperfusion model of spinal cord injury. Rat spinal cord slices were prepared and then exposed or not to oxygen deprivation and low glucose (ODLG) for 30, 45, 60, 75 and 90 minutes. Cell viability was assessed by triphenyltetrazolium (TTC), lactate dehydrogenase (LDH) release, and fluorochrome dyes specific for cell ...

background: the aim of this study was to investigate the preventive effect of the polyamine component n, n, n´, n´-tetrakis(2-aminoethyl)2,2-dimethylpropane-1,3-diamine (tdmtn) on apoptotic motor neurons in adult mouse spinal cord slices. materials and methods: thoracic region of adult mouse spinal cords was sliced by a tissue chopper into 400 μm slices and cultured in medium in the presence or...

OBJECTIVE We sought to define molecular and cellular participants that mediate motor neuron injury in amyotrophic lateral sclerosis using a coculture system. METHODS We cocultured embryonic stem cell-derived motor neurons with organotypic slice cultures from wild-type or SOD1G93A (MT) mice. We examined axon lengths and cell survival of embryonic stem cell-derived motor neurons. We defined and...

We present two approaches for automatically segmenting the spinal cord/canal from native CT images of the thorax region containing the spine. Different strategies are included to handle images where only part of the spinal column is visible. The algorithms require one seed point given on a slice located in the middle region of the spine, and the rest is automatic. The spatial extent of the spin...

Among various strategies employed for spinal cord injury, stem cell therapy is a potential treatment. So far, a variety of stem cells have been evaluated in animal models and humans with spinal cord injury, and epidermal neural crest stem cells represent one of the attractive types in this area. Although these multipotent stem cells have been assessed in several spinal cord injury models by ind...

Cannabinoids reduce tremor associated with motor disorders induced by injuries and neurodegenerative disease. Here we show that this effect is mediated cannabinoid receptors on astrocytes in the ventral horn of spinal cord, where alternating limb movements are initiated. We first demonstrate reduced a mouse model essential after intrathecal injection analog WIN55,212-2. investigate underlying m...

INTRODUCTION: The ability to non-invasively and quantitatively assess myelin content in the spinal cord would improve our understanding of a variety of spinal cord-related conditions, as well as enhance our ability to differentially diagnose, treat and monitor them. Currently, little is known about the pathology of myelin in spinal cord disease due to the technical challenges of measuring myeli...

cortical spreading depression (csd) has been suggested to underlie some neurological disorders such as migraine. despite the intensity with which many investigators have studied sd in the brain, only a few studies have aimed to identify sd in the spinal cord. here we described the main characteristic features of sd in the spinal cord induced by different methods including various spinal cord in...