Microsatellites or simple sequence repeats (SSRs) are very effective molecular markers in population genetics, genome mapping, taxonomic study and other large-scale studies. Variation in number of tandem repeats within microsatellite refers to simple sequence length polymorphism (SSLP); but there are a few studies that are showed SSRs replication slippage may be occurred during in vitro amplifi...

Consensus sequences of the Rattus norvtgicus Bland B2 repeats. During our sequence analysis of the flanking and intronic regions of six rat (Rattus norvqlcua) gamma-cryatallin genes, we encountered a number of repetitive elements closely reltted to either the murine Alu Bl or B2 type repeats (1,2). To our Knowledge no consensus sequence of either rat repeat has been published. We report here th...

results a number of 7-11 repeats in the spa gene xr region were determined. strains with 10 repeats were65%, therefore they had the highest percentage in isolates. seven repeats strains were 20% and each of the strains with 8, 9 and 11 repeats had the frequency of 5%. s. aureus strains antibiotic resistance was 35%, 5%, 45% and 40% for tetracycline, amoxicillin, gentamicin and erythromycin resp...

Length variation in short tandem repeats (STRs) is an important family of DNA polymorphisms with numerous applications in genetics, medicine, forensics, and evolutionary analysis. Several major diseases have been associated with length variation of trinucleotide (triplet) repeats including Huntington's disease, hereditary ataxias and spinobulbar muscular atrophy. Using the reference human genom...

Repetitive sequences are common not only in eukaryotic genomes but also in prokaryotic genomes. Bacterial genomes contain many types of repeats including tandem repeats and short dispersed repeats. This research aims to expand our understanding of short dispersed repeats (SDR), a novel type of repetitive sequences in cyanobacterial genomes. SDR are found in the genomes of Nostoc punctiforme as ...

Two independent isolates of a Bordetella pertussis repeated DNA unit were sequenced and shown to be an insertion sequence element with five nucleotide differences between the two copies. The sequences were 1053 bp in length with near-perfect terminal inverted repeats of 28 bp, had three open reading frames, and were each flanked by short direct repeats. The two insertion sequences showed consid...

Many new classes of Polymerase Chain Reaction (PCR) -based genetic markers have been developed over the last decade. Of these, microsatellite markers are widely regarded as one of the most useful identifi ed so far1,2,3. The signifi cance of microsatellite markers derives from their abundance in the genome, single locus nature, simplicity of assay, high levels of allelic diversity, mendelian in...

Microsatellites (SSR--simple sequence repeats, STR--short tandem repeats, SSLP--simple sequence length polymorphism, VNTR--variable number of tandem repeats) are the class of repetitive DNA sequences present in all living organisms. Particular characteristics of microsatellites, such as their presence in the genomes of all living organisms, high level of allelic variation, co-dominant mode of i...