Data on 324 Holstein bulls from the July 1983 USDA Sire Summary and Holstein Association type evaluations were analyzed using multiple regression to determine which traits or combination of traits in a sire evaluation are most important in determining the price of a straw of semen. Over all bull studs, type was a major economic influence in determining price of semen. When bulls with outlier pr...

This study was carried out to obtain informations regarding the effect of N-acetyl-D-glucosamine in the LEY (lactoseegg yolk) diluent according to incubation time in 5 ml maxi-straw and the effects of freezing rate, thawing temperature and thawing time in the LEN (lactose-egg yolk and N-acetyl-D-glucosamine) diluent on acrosome morphology and motility of frozen-thawed boar sperm. The study show...

Three experiments were conducted to test fertility when sperm numbers per insemination ranged from 10 x 10(6) to 40 x 10(6) total sperm. All semen was from Holstein bulls that were on a regular schedule of semen collection. The semen was extended with heated homogenized whole milk, cooled, glycerolated, and frozen according to standard procedures. Semen was distributed to a large group of insem...

The objective of the present study was to establish an effective semen freezing procedure to set up a cryobank for chicken breeds and genetic lines of special interest. The fowls used in the experiments originated from White Leghorn lines maintained as conservation flocks. In this study, different protocols were evaluated in terms of differences in the fertilization success. 1 A) Comparison of ...

Clinical embryo vitrification evolved with the development of unique vitrification devices in the 21st century and with the misconception that ultra-rapid cooling in an "open" system (i.e., direct LN2 contact) was a necessity to optimize vitrification success. The dogma surrounding the importance of cooling rates led to unsafe practices subject to technical variation and to the creation of vitr...

In cynomolgus macaques, an important animal species for biomedical research, efficient reproduction has been hampered partly due to the difficulties of artificial insemination (AI) using straw tubes developed for humans or farm animals, because cynomolgus macaques have a complex cervical canal structure. In this study, taking into consideration the unique structure of the macaque cervical canal...

Cryopreservation of boar semen is still considered suboptimal due to lower fertility as compared with fresh samples when glycerol, a permeating cryoprotectant, is used. Trehalose is a non-permeable cryoprotectant and nonreducing disaccharide known to stabilize proteins and biologic membranes. The aim of this study was to evaluate the cryosurvival and in vitro penetrability of boar spermatozoa w...

In the present study, three experiments were designed to identify most appropriate technique for freezing rabbit semen. Experiment 1 aimed determine optimal levels of dimethyl sulfoxide (DMSO) contents in medium and their effects on individual bucks. Semen ejaculates each buck (n=15 bucks) mixed split into portions extension with a containing varying concentrations DMSO (0.75, 1.0, 1.4 M). Dilu...

Isolation of high-quality RNA is important for assessing sperm gene expression, and semen purification methods may affect the integrity of the isolated RNA. This study evaluated the effectiveness of the sperm swim-up method for seminal RNA isolation. Frozen semen samples in straws from three bulls of proven fertility were purified by the swim-up method. RNA extraction was carried out using the ...

BACKGROUND Except for testicular cancer and Hodgkin's disease, baseline data on semen quality in case of cancers as well as systemic pathologies of the young adult are scarce or based on low sample size. METHODS Semen quality in patients having testicular cancer (TGCT, n = 2315), Hodgkin's disease (HD, n = 1175), non-Hodgkin's lymphoma (NHL, n = 439), leukemia (L, n = 360), sarcoma (S, n = 20...