1 The abbreviations used are: KDO, 3-Deoxy-D-manno-octulosonate; KDO 8-P, 3-DeoxyD-manno-octulosonate 8-phosphate; Tris, Tris-(hydroxymethyl)aminomethane; HEPES, N-[2Hydroxyethyl]piperazine-N’-[2-ethanesulfonic acid]; PNPase, purine nucleoside phosphorylase; EDTA, (ethylenedinitrilo)tetraacetic acid; 2D gel electrophoresis, two dimensional gel electrophoresis; SDS-PAGE, sodium dodecyl sulfate-p...

labeled membrane proteins by density gradient ultracentrifugation using 1 O%-40% sucrose gradients containing the nonionic detergent. Studies were performed using soluble proteins derived from membranes isolated in the presence or absence of EDTA. Analysis of gradient fractions by SDS-polyacrylamide gel electrophoresis showed that in the absence of divalent cation chelation. GP lIb and lIla pen...

labeled membrane proteins by density gradient ultracentrifugation using 1 O%-40% sucrose gradients containing the nonionic detergent. Studies were performed using soluble proteins derived from membranes isolated in the presence or absence of EDTA. Analysis of gradient fractions by SDS-polyacrylamide gel electrophoresis showed that in the absence of divalent cation chelation. GP lIb and lIla pen...

Alkaline proteases are the most important group of industrial enzymes that hydrolyse peptide bond proteins into small peptides. The demand alkaline protease predominantly from microbial origin has been recently increased and enhances research for with high stability at extreme conditions. Thus, this study is aimed to characterize bacterial isolate, Bacillus subtilis VBC7 screened dairy waste du...

In the present study purification and characterization of a novel extracellular pullulanase enzyme from Bacillus thuringiensis was carried out for use in detergents. Maximum production (5.71 U/ml) obtained medium containing tryptone as carbon energy source having pH 6.0 when it inoculated with 3% overnight grown inoculum incubated at 37°C 24 hrs.  Optimal conditions pullalanse activity wer...

A protease which was activated by SDS was purified to homogeneity from maize leaves. On the basis of its proteolytic activity towards ribulose-1,5-bisphosphate carboxylase/ oxygenase (Rubisco) or a synthesized peptide, the purification was carried out using immunoaffinity chromatography with a monoclonal antibody raised against a partially purified enzyme by native gradient PAGE. The purified p...

Cell extracts from Pyrococcus furiosus were found to contain five proteases, two of which (S66 and S102) are resistant to sodium dodecyl sulfate (SDS) denaturation. Cell extracts incubated at 98 degrees C in the presence of 1% SDS for 24 h exhibited substantial cellular proteolysis such that only four proteins could be visualized by amido black-Coomassie brilliant blue staining of SDS-polyacryl...

Source/Description: The polymerase chain reaction (PCR) and allele specific oligonucleotide (ASO) hybridisation were used to detect a common polymorphism within the open reading frame (ORF) of the human prion protein (PRIP) gene. This polymorphism has previously been reported as a mutation (1). The oligonucleotide sequences for amplification of the ORF of the PRIP contained EcoRI and BamHI link...

Formalin is the most commonly used fixative for light microscopy because of its preservation of -morphological details. A major adverse effect of formalin fixation is formation of cross-linkages between epitopes (amino acid residues) and unrelated proteins by formaldehyde groups. The great majority of monoclonal and polyclonal antibodies used for immunohistochemical (IHC) staining of formalin-f...