rosine B, Tartrazine, Lissamine Green B, Pyronin G, Acetyl Yellow G, Ponceau 6R, Ponceau SX, Food Orange #2, and Food Yellow #3. The concentration of dye tested was 50 mg/ L of incubation mixture (25 pg/tube) in RIA systems for human lutropin (hLH), its alpha and beta subunits, rat LII, rat corticotropin (rACTH), rat insulin, and human thyroxin (T4) and testosterone. (For T4 we usedthe MAGIC-T4...

In this paper, we report the successful synthesis of metal ion-doped TiO(2) nanoparticles via a simple solution combustion method employing a mixture of ethanol and ethyleneglycol (v/v = 30/20) as the solvent, tetra-n-butyl titanate [Ti(OC(4)H(9))(4), TBOT] as the titanium source and oxygen gas in the atmosphere as the oxygen source, in the presence of small amounts of metal ions such as Cu(2+)...

CARDIAC histology has been reviewed in 16 patients between April 1980 and March 1981 to evaluate the effect of cyclosporin-A (CyA) and low-dose prednisone in a new trial of orthotopic cardiac transplantation. The usual maintenance dose of CyA has been 5-10 mg/kg/day, and prednisone 15-20 mg/ day.1 HydrOC()rtisone, 1 g intravenously, has been used to combat rejection. usually without alteration ...

The 2 techniques are based upon the formulation of lead ing ion and trailing ion initially enunciated by Ornstein (3) and lean heavily on the suggestions for buffer strategy made by Williams and Reisfeld (6). The leading ion is po tassium, the trailing ion is fl-alanine in the pH 3.8 system or 3-picoline, (3-methylpyridine) (pK 6.29) in the pH 6.0 system. Acetic acid and cacodylic acid (pK 6.4)...

1. Basic dyes (methyl green/pyronin, and toluidin blue), in conjunction with ribonuclease, may be used to detect the cytochemical localization of ribonucleic acid. This technique has been examined critically. 2. The fixative used is important and results vary with different materials. It is suggested that Zenker and Serra fixation should be compared in preliminary investigations. Unexplained an...

A MUTATION of Xenopus has been found which reduces the maximum number of nucleoli per diploid nucleus from 2 in the wild-type to 1 in heterozygotes. Homozygous mutants possess no true nucleoli, hence being termed anucleolate. They do, however, possess pyronine-staining intranuclear organelles that are smaller and more numerous than typical nucleoli. The mutation can be considered as a recessive...

Although rhodamine dyes have been extensively studied for a variety of applications, many details of their photophysics are not yet fully understood, including the possible presence of a charge separated electronic state lying near the optically active excited singlet state and the role of twisting substituent groups in excited-state quenching. To address this, a large library of rhodamine dyes...

A study has been made of the relationship of antibody formation and the changes in amount of the nucleic acids in rabbit lymph nodes draining areas injected with typhoid vaccine. The increase in DNA was found to parallel the increase in weight of the nodes, as might be expected from the active multiplication of cells. The peak of PNA increase occurred between the 4th and 6th days after vaccine ...

Hydropersulfide (R-SSH) is an important class of reactive sulfur species (RSS) involved in a variety of physiological processes in mammals. A fluorescent probe capable of real-time detection of hydropersulfide levels in living cells would be a versatile tool to elucidate its roles in cell signalling and redox homeostasis. In this paper, we report a ratiometric fluorescent probe for hydropersulf...

Hydropersulfide (R–SSH) is an important class of reactive sulfur species (RSS) involved in a variety of physiological processes in mammals. A fluorescent probe capable of real-time detection of hydropersulfide levels in living cells would be a versatile tool to elucidate its roles in cell signalling and redox homeostasis. In this paper, we report a ratiometric fluorescent probe for hydropersulf...