Oral administration of 1 ml of 3.42 M NaCl solution to rats induced spermidine/spermine N 1-acetyltransferase (SSAT) activity in gastric mucosa as well as ornithine decarboxylase (ODC) activity. SSAT activity increased and peaked at 5 h and again at 7 h, whereas ODC activity peaked at 6 h. SSAT mRNA also increased after 3.42 M NaCl administration to an extent similar to the increase in SSAT act...

The administration to rats of putrescine (750 mumol/kg body wt.) caused in liver, kidney and heart an increase in putrescine at 1 h and in diamine oxidase (EC 1.4.3.6) activity within 3-6 h. An increase in spermidine was observed at 9 h in liver and at 6 h in kidney, whereas in heart there was no change. The increase in diamine oxidase activity by exogenous putrescine was prevented by the admin...

Putrescine uptake and the kinetics of this uptake were studied in petals of Saintpaulia ionantha Wendl. Uptake experiments of [(3)H] or [(14)C] putrescine were done on single petals at room temperature at various pH values. The results show that putrescine uptake occurs against a concentration gradient at low external putrescine concentration (0.5-100 micromolar) and follows a concentration gra...

Putrescine (diaminobutane) was previously found to stimulate proliferation of human fibroblasts in tissue culture, and a growth factor produced by these cells was identified as putrescine. In the present paper putrescine transport is studied. The rate of putrescine transport was dependent on temperature, and most of the labeled putrescine was retained by the cells after washing with excess unla...

BACKGROUND The effects of nitrite (0, 100, and 200 mg kg(-1)) and nisin (0, 250, and 500 mg kg(-1)) on biogenic amine formation in sucuk were investigated by utilising a central composite design of response surface methodology. RESULTS The addition of nitrite led to decreased levels of tryptamine, 2-phenylethylamine, putrescine, cadaverine, tyramine, and histamine, whereas nisin decreased the...

The mechanism of mammalian polyamine transport is poorly understood. We have investigated the role of plasma-membrane potential (DeltaPsipm) in putrescine and spermidine uptake in ZR-75-1 human breast cancer cells. The rate of [3H]putrescine and [3H]spermidine uptake was inversely correlated to extracellular [K+] ([K+]o) and to DeltaPsipm, as determined by the accumulation of [3H]tetraphenylpho...

in order to study the effect of pre and postharvest polyamines spraying on increasing vase life of alstroemeria (alstroemeriaaurantica cv.sukari), an experimental was conducted on completely randomized factorial design with two types polyamines (putrescine, spermine) in four levels (zero, five, 10, 15 and 20 mg/l). results showed that, 20 mg/l of putrescine and spermine treatments increased vas...

The only pathway known for the biosynthesis of sperrnidine is from putrescine and S-adenosylmethionine. S-Adenosylmethionine is decarboxylated, and the aminopropyl group of the product is transferred to putrescine to form spermidine. The enzymes of this pathway, S-adenosylmethionine decarboxylase (EC 4.1.1 S O ) and aminopropyltransferase (EC 2.5.1.16), have been found in a number of bacteria, ...

The rate of uptake of putrescine by Anacystis nidulans has been shown to depend on the external pH and the extracellular concentration of putrescine. Accumulation of exogenous putrescine was also proportional to the concentration of putrescine in the medium, suggesting that putrescine uptake was not subject to cellular regulation. An equation was derived to test the hypothesis that putrescine a...

In this study investigated the effect of putrescine sprayed (0.5 & 1mM) on Chamomile (Matricaria chamomilla L.) and the changed of Anthocyanin, Phenolic compounds, POX & CAT Antioxidative enzymes were examined. The results show that Putrescine treatments increased hydrogen peroxide in seedlings to compare control. Phenolic compound content under putrescine treatments are different. Phenolic com...