The publication by Dorji et al. [1], “Microscopic Image of Leishman-Donovan Bodies in Bone Marrow Aspirate Smear of Patient Suffering from Unexplained Intermittent Low-Grade Fever and Cough”, is very interesting. Indeed, the clinical presentation of leishmaniasis is usually nonspecific and it is hard to discriminate it from other tropical infections [2]. However, the common presentation that mi...

The IgM mAbs 12A1 and 13F1 are protective and nonprotective, respectively, against lethal Cryptococcus neoformans infection in mice. To better understand the variables that contribute to IgM efficacy against C. neoformans, we studied the effects of inoculum size, route of infection, and Ab dose for each of these mAbs. mAb 13F1 did not prolong survival under any condition studied. mAb 12A1 prolo...

The incidence of a high percentage of negative rose bengal test (~BT~ sera which showed a high titre in the serum agglutmat10n (SAT) and complement fixation tests. (CFf). was noted in certain batches of sera during routme testmg for bo~ine brucellosis. As a similar phe~omenon has not previously been recorded, an investigation was undertaken to establish whether in this case a prozone reaction l...

Three cases of false-negative cerebrospinal fluid latex agglutination test results for patients with culture-positive cryptococcal meningitis are reported. False-negative results occurred in settings of low cryptococcal antigen concentrations in cerebrospinal fluid and were dependent on the latex agglutination test kit used. Investigation of each case revealed that prozone phenomena or interfer...

A panel of monoclonal antibodies that bind to leukemic blast cells from patients with acute myelocytic leukemia and chronic myelocytic leukemia in blast crisis was studied for their ability to mediate complement-dependent lysis of a variety of cell populations from patients with leukemia, normal blood cells, and human leukemia cell lines. Several of these monoclonal antibodies were selectively ...

Abstract Objective The aim of this study is to evaluate the analytical performances following Sentinel Diagnostics’ assays: Total Bile Acid Liquid, Copper, Aldolase, on Roche cobas c 303 unit. Methods Performance evaluation included Limit Blank (LoB), Detection (LoD), Quantitation (LoQ), linearity, intra assay precision, inter board reagent stability, prozone effect, instrument correlation curr...

Since the publication of the original complementfixation test for syphilis by Wassermann, Neisser, Bruck, and Schucht (1906) and the publication of the flocculation test of Porges and Meier (1908), innumerable modifications have been described for the improvement of both diagnostic methods. The technical complexity of the former furnished an incentive for the development of the latter. Yet, des...

A rational strategy of magnetic relaxation switches was proposed here to detect a(1)-acid glycoprotein (AGP), an acute phase a-globulin plasma glycoprotein. The assay was based on the relaxation time change between the aggregation of magnetic nanoparticles with concanavalin A and the redispersion with AGP, which can avoid the prozone effect and improve the detection accuracy. The assay was an e...