The distinct reproductive program of orchids provides a unique evolutionary model with pollination-triggered ovule development and megasporogenesis, a modified embryogenesis program resulting in seeds with immature embryos, and mycorrhiza-induced seed germination. However, the molecular mechanisms that have evolved to establish these unparalleled developmental programs are largely unclear. Here...

To screen for suitable conditions cluster bud induction of Cremastra appendiculata . The aseptic germination protocorms of C.  seeds were used as explant materials to explore the effects different basic media, types plant growth regulators, anti-browning agents and their concentrations on buds of results showed that: 1/4MS medium was buds growing; when concentratio...

The main purpose of this article is to review role several organic growth additives such Apple juice, coconut water (CW), maize extract, banana homogenate (BH), peptone and protocorms etc which stimulate the multiplication rate various orchid species in vitro multiplication. These supplements help increase number shoots, root leaf culture medium. In many tissue culture, supplements, are most es...

an in vitro plant regeneration protocol was successfully established in aphyllorchis montana , a saprophytic achlorophyllous orchid by culturing immature seeds. among the six basal media evaluated for seed germination, bm-tm medium was found to be the best followed by kc medium. after 40 days, all the media turned brown and the growths of the protocorms were arrested. activated charcoal, 1 g/l ...

The population of many splendid orchids is reducing from their natural habitats at an alarming rate and their conservation is becoming a matter of global concern. Asymbiotic seed germination has been applied for ex situ conservation of rare, endangered and threatened orchid taxa and could provide rapid means their multiplication. In the present study reported here, seeds of an epiphytic and rar...

The paper presents the results of study temperature regime optimization for storing seeds tropical orchids on example Eulophia streptopetala Lindley and Stanhopea tigrina Batem ex Lindl. Mature were frozen at temperatures 18C, 40C or 80C a period 1, 3, 6 12 months, storage was +4C. Before after freezing, viability determined by visual tetrazole methods. After defrosting, sown plants grown from ...