objective: in all protocols for isolation of mesenchymal stem cells (mscs), a few days after culture initiation, the medium were discarded along with its contents of non-adherent cells and the adherent cell population kept and expanded as mscs population. in the present study, attempt was made to expand the cells suspended in removed medium of primary culture and compare them with the adherent ...

Hippocampal neural stem/progenitor cells (hipp-NS/PCs) of the adult mammalian brain are important sources of neuronal and gial cell production. In this study, the main goal is to investigate the plasticity of these cells in neuronal/astroglial differentiations. To this end, the differentiation of the hipp-NS/PCs isolated from 3-month-old Wistar rats was investigated in response to the embryonic...

Herein, we presented the first report on bluetongue (BT) disease in 10 pregnant camels in a herd fromKerman province, Iran. All sera samples were tested serologically (AGID, C-ELISA). We also used the Razi-BK cell line, performed primary culture of ovine kidney and inoculated intravenously the embryonatedchicken eggs (ECE) to culture and isolate the BT virus. Efforts to culture and isolation of...

conclusions our underlying hypothesis is that recapitulating the three-dimensional early embryonic structure, in order to reproduce better in vitro the three-dimensional morphogenetic-like re-arrangements, would improve cells differentiation, when in vivo transplanted; moreover, it could be used as a simplified cancer disease model and reliable drug evaluation method as well. results the proces...

we have refined the technique for isolating and propagating cultures of primary ovarian carcinosarcoma cells (oscs) derived from ascites, which allowed the cells to obtain the biphasic features of carcinosarcoma in cell culture conditions (presence of both carcinoma and mesenchymal morphologic types). this protocol involves a simple yet rapid method for the growth and propagation of ascites osc...

We have used culture conditions which simulate the microenvironment of breast tumors for the isolation and propagation of primary breast tumor cells in vitro. In this monolayer setup, the mixture of cells dissociated from primary breast tumors is subjected to self-created gradients of oxygen and nutrients as well as metabolic waste and extracellular pH. The tumor populations isolated under thes...

We have used culture conditions which simulate the microenvironment of breast tumors for the isolation and propagation of primary breast tumor cells in vitro. In this monolayer setup, the mixture of cells dissoci ated from primary breast tumors is subjected to self-created gradients of oxygen and nutrients as well as metabolic waste and extracellular pH. The tumor populations isolated under the...

results aβ (1-42) stimulated the production of the proinflammatory cytokines il6, il1β, tnfα, and il18 in both the primary microglia cell culture and the b92 cell line. both the raav-il4 construct and the ril-4 protein were found to inhibit production of the most important aβ (1-42)-induced proinflammatory cytokine mrnas in the two types of cells with different patterns. conclusions it seems th...