Metal (Me) and MeATP interactions with adenylate cyclases associated with rabbit ventricular particles and with a detergent-dispersed preparation from rat cerebellum have been studied. data were simulated to fit kinetic models in which an inhibitor (HATP or ATP) is added in constant proportion to the variable substrate (MeATP). The specific models considered were that the enzyme binds (a) MeATP...

Pepstatin has been shown to be an extremely potent inhibitor of human renin (Ki = 1.3 X lo-lo M). Kinetic analyses by several techniques show reversible interaction of pepstatin with renin and classical noncofipetitive inhibition. The high affinity of pepstatin for renin allows kinetic determination of the molarity of the enzyme in partially purified preparations. This method has been validated...

Because of the importance of developing stereoselective syntheses for single enantiomers, a selected panel of racemic biologically active 1-aryl- and 1-heteroarylpropan-2-amines has been prepared, followed by a study of their behavior in enzymatic kinetic resolution (KR) processes. For this purpose, lipase B from Candida antarctica (CAL-B) proved to be an ideal biocatalyst allowing the preparat...

Pepstatin has been shown to be an extremely potent inhibitor of human renin (Ki = 1.3 X lo-lo M). Kinetic analyses by several techniques show reversible interaction of pepstatin with renin and classical noncofipetitive inhibition. The high affinity of pepstatin for renin allows kinetic determination of the molarity of the enzyme in partially purified preparations. This method has been validated...

The synthesis of chiral amines is still a challenge for organic synthesis since optically pure amines are of great importance for the pharmaceutical and agrochemical industries. Among all the methodologies developed until now, chemoenzymatic dynamic kinetic resolution has proven to be useful for the preparation of enantioenriched primary chiral amines. In our continuous efforts toward the devel...

A kinetic priority queue is a kinetic data structure which determines the largest element in a collection of continuously changing numbers subject to insertions and deletions. Due to its importance, many different constructions have been suggested in the literature, each with its pros and cons. We propose a simple construction that takes advantage of randomization to achieve optimal locality an...

The substrate selectivities of an anti-phosphonate and an anti-phosphate kinetically homogeneous polyclonal catalytic antibody preparation and two hydrolytic enzymes were compared by using hapten-analogous and truncated carbonate and ester substrates each containing a 4-nitrophenolate leaving group. Syntheses of the truncated substrates devoid of recognition features in the non-leaving group pa...

1. Measurements of kinetic constants for a purified preparation of human-placenta oestradiol dehydrogenase have been made. 2. These constants have been compared with similar measurements made on crude ammonium sulphate precipitates of human-placenta homogenates. The comparison indicates that nearly half of the observed nicotinamide nucleotide-transhydrogenation activity in the crude preparation...

The kinetic behaviour of humidity sensors based on a cross-linked interpenetrated polyelectrolyte was investigated. Various parameters were changed in order to study their effect on the response to a humidity variation. The influence of the way of preparation of the polymer and of the sensing layer thickness are reported and discussed.

The preparation of chiral alcohols and amines by using iridium catalysis is reviewed. The methods presented include the reduction of ketones or imines by using hydrogen (hydrogenations), isopropanol, formic acid, or formate (transfer hydrogenations). Also dynamic and oxidative kinetic resolutions leading to chiral alcohols and amines are included. Selected literature reports from early contribu...