نتایج جستجو برای: polya pcr

تعداد نتایج: 175977  

Journal: :Gene 1999
J E Tabaska M Q Zhang

We present polyadq, a program for detection of human polyadenylation signals. To avoid training on possibly flawed data, the development of polyadq began with a de novo characterization of human mRNA 3' processing signals. This information was used in training two quadratic discriminant functions that polyadq uses to evaluate potential polyA signals. In our tests, polyadq predicts polyA signals...

2007
George Pólya

The undisputed father of mathematical problem solving is George Pólya (December 13, 1887 – September 7, 1985), one of the giants of classical analysis in the 20 century. He considered solving problems to be a practical art, one that can be taught and learned. His books on the subject How To Solve It (1945) and the two-volume set Mathematics and Plausible Reasoning (1954), and Mathematical Disco...

Journal: :Clinical chemistry 2005
Nurith Kurn Pengchin Chen Joe Don Heath Anne Kopf-Sill Kathryn M Stephens Shenglong Wang

BACKGROUND Global analysis of the genome, transcriptome, and proteome is facilitated by the recent development of tools for large-scale, highly parallel analysis. We describe a novel nucleic acid amplification system that generates products by several methods. 3'-Ribo-SPIA primes cDNA synthesis at the 3' polyA tail, and whole transcript (WT)-Ribo-SPIA primes cDNA synthesis across the full lengt...

Journal: :Molecular and cellular biology 2005
Kathryn E Plant Michael J Dye Celina Lafaille Nick J Proudfoot

The human gamma-globin genes form part of a 5-kb tandem duplication within the beta-globin gene cluster on chromosome 11. Despite a high degree of identity between the two genes, we show that while the upstream Ggamma-globin gene terminates transcription efficiently, termination in the Agamma gene is inefficient. This is primarily due to the different strengths of the polyA signals of the two g...

2012
Jean-François Noël Stéphanie Larose Sherif Abou Elela Raymund J. Wellinger

The RNA component of budding yeast telomerase (Tlc1) occurs in two forms, a non-polyadenylated form found in functional telomerase and a rare polyadenylated version with unknown function. Previous work suggested that the functional Tlc1 polyA- RNA is processed from the polyA+ form, but the mechanisms regulating its transcription termination and 3'-end formation remained unclear. Here we examine...

2014
Jean-Claude Debouzy David Crouzier Fréderic Bourbon Malika Lahiani-Skiba Mohamed Skiba

The properties of an amorphous solid dispersion of cyclosporine A (ASD) prepared with the copolymer alpha cyclodextrin (POLYA) and cyclosporine A (CYSP) were investigated by (1)H-NMR in solution and its membrane interactions were studied by (1)H-NMR in small unilamellar vesicles and by (31)P (2)H NMR in phospholipidic dispersions of DMPC (dimyristoylphosphatidylcholine) in comparison with those...

2012
Keiko Miyadera Ian Brierley Jesús Aguirre-Hernández Cathryn S. Mellersh David R. Sargan

UNLABELLED Mutations in RPGRIP1 are associated with early onset retinal degenerations in humans and dogs. Dogs homozygous for a 44 bp insertion including a polyA(29) tract potentially leading to premature truncation of the protein, show cone rod degeneration. This is rapid and blinding in a colony of dogs in which the mutation was characterised but in dogs with the same mutation in the pet popu...

2012
LIJUN SUN YUEWEN HAO XIAOWEI NIE XUEXIN ZHANG GUANGXIAO YANG QUANYING WANG

The objective of this study was to investigate the effect of the PR39 recombinant adeno-associated virus (AAV) controlled by the hypoxia-responsive element (HRE) on gene therapy of ischemic heart disease. The minimal HRE was artificially synthesized and the AAV vector controlled by HRE was introduced with NT4-TAT-His-PR39 to investigate the expression of AAV-PR39 in hypoxic vascular endothelial...

2001
Matti Pitkänen

Hilbert-Polya conjecture and Riemann hypothesis are proven. The construction of Hilbert-Polya operator is inspired by the conviction that Riemann Zeta function is associated with a physical system allowing superconformal transformations as its symmetries. The proof as such is elementary involving only basic facts about the theory of Hilbert space operators and complex analysis.

Journal: :BioTechniques 2000
F S Celi D Mentuccia L Proietti-Pannunzi C R di Gioia M Andreoli

PCR-based strategies such as competitive RT-PCR or semiquantitative RTPCR are commonly used for quantitating low-abundance mRNA transcripts (4,7–9). Competitive RT-PCR relies on amplifying the same target with a known amount of a competitor/reference sequence and comparing the relative amounts of the two PCR products. To improve the sensitivity and accuracy of these methods, often expensive and...

نمودار تعداد نتایج جستجو در هر سال

با کلیک روی نمودار نتایج را به سال انتشار فیلتر کنید