The virulence plasmid-carried apy (phoN2) gene of Shigella and related enteroinvasive Escherichia coli (EIEC) encodes apyrase, an ATP-diphosphohydrolase belonging to class A of the non-specific acid phosphatases (A-NSAPs). Apyrase and A-NSAPs share three domains of conserved amino acids (domains D1-D3) containing residues forming the putative active site of apyrase. In spite of their similarity...

A comparative study of various treatments for inhibition of the residual activity of a lipase (obtained from Bacillus coagulans MTCC-6375) in a colorimetric assay using p-nitrophenyl palmitate (pNPP) was made. Direct chilling of contents of reaction mixture or addition of chilled mixture of ethanol : acetone (1:1) decreased the residual lipase activity by 94.0 and 95.0% respectively, as compare...

BACKGROUND The isozymes of alkaline phosphatase, the tissue non-specific, intestinal and placental, have similar properties and a high degree of identity. The placental isozyme (PLAP) is an oncofetal antigen expressed in several malignancies including choriocarcinoma, seminoma and ovarian carcinoma. We had earlier attempted to isolate PLAP-specific scFv from a synthetic human immunoglobulin lib...

Materials and Methods: The stable state kinetic for comparison of inhibitory effect of artimisinin, dihydroartimisinin and cyclosporine A on calcineurin activation was used in this study. First, the best inhibitory concentration of artimisinin, dihydroartimisinin and cyclosporine A was calculated. Afterwards, the km and Vmax of them in the presence of substrate, paranitrophenylphosphate (PNPP) ...

The effect of Tris, Glycine and Tricine on alkaline phosphatase activity was tested at a pH of 8.6, 8.85 and 9.1, using pNPP as substrate. Alkaline phosphatase activity was measured by monitoring the increase in NP absorbance at 420nm using a spectrophotometer. Alkaline phosphatase in Tris buffer at pH 9.1 was found to have the highest activity. MichaelisMenten enzyme kinetic analysis revealed ...

The following Supporting Methods describe determination of the charge density on each nonbridging oxygen atom and catalytic proficiencies for the R166S AP-catalyzed reactions of the following substrates: para-nitrophenyl phosphate (pNPP), para-nitrophenyl phosphorothioate (pNPPS), bis-(para-nitrophenyl) phosphate (bpNPP), methyl para-nitrophenyl phosphate (MpNPP), methyl para-nitrophenyl phosph...

Abstract. In this study, we investigate the maximum physical and biogeochemical potential of macroalgae open-ocean mariculture sinking (MOS) as an ocean-based carbon dioxide removal (CDR) method. Embedding a model into Earth system model, simulate in surface layer followed by fast carbon-rich macroalgal biomass to deep seafloor (depth>3000 m), which assumes no remineralization harvested duri...

Phosphoesterases are involved in the degradation of organophosphorus compounds. Although phosphomonoesterases and phosphotriesterases have been studied in detail, studies on phosphodiesterases are rather limited. In our search to find novel phosphodiesterases using metagenomic approach, we cloned a gene encoding a putative phosphodiesterase (PdeM) from the metagenome of the formation water coll...

The aim of this work was to determine the kinetic properties and evaluate the effect of some flavonoids on human lymphocyte protein tyrosine phosphatase. Tyrosine-, serineand threonine-phosphate were hydrolyzed by this phosphatase 60%, 20% and 10%, respectively. In the kinetic studies the enzymatic activity was determined by using p-nitrophenylphosphate (pNPP) as substrate. The enzyme presented...