Protein kinases I (PK I) and II (PK II) were purified 253- and 13.5-fold, respectively, from an extract of sonically disrupted cells of Legionella micdadei by ion-exchange chromatography on QAE-Sephadex, by histone affinity chromatography, and by HPLC-gel filtration chromatography. Both enzymes catalysed the phosphorylation of calf thymus histones, with a Km of 2.7 mg ml-1 for PK I and 2.9 mg m...

The acidic dissociation constants in the range H,, -1.3 to pH 7 of folic acid, dihydrofolic acid, methopterin (N(lO)methylfolic acid), and methotrexate have been measured by potentiometric and spectrophotometric titrations. Assignment of these dissociations was made by comparison to model compounds, by proton magnetic resonance measurements, and by examination of associated ultraviolet absorban...

Mature erythrocytes contain a specific isozyme of pyruvate kinase (R-PK) while immature erythroblasts coexpress R-PK and another isozyme, M2-PK. To determine what roles degradation and decreasing of synthesis played in the disappearance of M2-PK during erythroid maturation, M2-PK and R-PK synthesis and degradation were studied in erythroblasts from fetal liver and blood BFU-E-derived erythrobla...

purpose: to investigate the biomechanical features of transplanted corneas after penetrating keratoplasty (pk), deep anterior lamellar keratoplasty (dalk), and descemet stripping automated endothelial keratoplasty (dsaek) and compare these measurements with those of a normal group. methods: forty-five eyes of 36 patients underwent pk, 23 eyes of 21 patients received dalk using anwar’s big bubbl...

purpose: to assess the efficacy and safety of laser in situ keratomileusis (lasik) for correction of residual myopia and astigmatism after penetrating keratoplasty (pk). methods: in this prospective interventional case series, lasik was performed on 14 eyes of 13 patients unable to wear glasses or contact lenses after pk. the interval between lasik and pk was at least one year. all patients had...

background: selection of the best sedative regimen during pediatric endoscopy with greater stability in hemodynamic parameters and fewer side effects is very important. the aim of this study was to compare the clinical efficacy and safety of propofol – ketamine versus propofol – fentanyl in pediatric undergoing diagnostic upper gastrointestinal endoscopy (ugie). methods: in this clinical trial,...

Objective(s) As there are conflicting findings regarding the clearance-dose and patient characteristics relationships for valproic acid (VPA), this study was conducted to investigate the relationship between patient demographic characteristics, VPA dosage and the drug clearance in adult Iranian patients. Materials and Methods Patients (N= 47) were either on monotherapy with VPA or were under co...

purpose: to compare the outcomes of deep anterior lamellar keratoplasty (dalk) using the big-bubble technique with that of penetrating keratoplasty (pk) in patients with keratoconus. methods: this double-blind clinical trial included patients with moderate to advanced keratoconus and poor spectacle-corrected visual acuity or contact lens intolerance. refractive errors, best corrected visual acu...