Elevated levels of tissue crosslinking are associated with numerous diseases (cancer stroma, organ fibrosis), and also eliminate the otherwise remarkable clinical successes of tissue-derived scaffolds, instead eliciting a foreign body reaction. Nevertheless, it is not well understood how the initial physical and biochemical properties of cellular microenvironments, stem cell niches, or of 3D ti...

Polyvinyl alcohol gelatin hydrogels were fabricated using genipin as a crosslinking agent for bone regeneration application. Optimized formulation of PVA-GE hydrogel was fabricated using genipin as crosslinking agent. Characterizations such as FTIR, morphology, porosity, pore size, degradation and swelling rate were investigated. Bone regeneration potential of optimized genipin cross-linked pol...

The mechanisms by which a cell uses and adapts its functional membrane organization are poorly understood and are the subject of ongoing investigation and discussion. Here, we study one proposed mechanism: the crosslinking of membrane components. In immune cell signaling (and other membrane-associated processes), a small change in the clustering of specific membrane proteins can lead to large-s...

showing the occurrence of glass/rubber transition in bovine serum albumin (a globular protein) atlow moisture levels and the occurrence of maillard crosslinking in gelatin in previous works, the importanceof the state of glass or rubber of bsa on the rate and extent of polymerisation, protein solubility, colour andsds page patterns in the presence and absence of ascorbic acid, diketogulonic aci...

Design of experiment is used to develop a hybrid methylcellulose hydrogel that combines physical and chemical crosslinks, resulting in an injectable, in situ stiffening, and long-lasting material with predictable swelling and rheological properties. Chemical crosslinking is complete prior to injection, allowing for ease of use and storage. Controlled release of two relevant protein therapeutics...

The 3D encapsulation of cells within hydrogels represents an increasingly important and popular technique for culturing cells and towards the development of constructs for tissue engineering. This environment better mimics what cells observe in vivo, compared to standard tissue culture, due to the tissue-like properties and 3D environment. Synthetic polymeric hydrogels are water-swollen network...