conclusions the rabies virus np could be extracted and purified in an appropriate amount from infected cell culture. the results of sds-page analysis showed that the intact rabies virus np had been purified properly and thus could be used for further steps to produce the specific diagnostic rabies conjugate. results the volume of the lysate was 15 ml and it became 2.5 ml after purification, wit...

The nucleic acids of tubercle bacilli have formed the subject of several studies, among which may be mentioned those by Ruppel (l), Levene (2), and Johnson and his associates (3-5). The work of Mensel and Heidelberger (6) on the fractionation of the proteins of the tubercle bacillus revealed the presence of several protein fractions, rich in phosphorus and in purines, which appeared to be nucle...

The X-ray crystal structure of the Lymphocytic choriomeningitis virus nucleoprotein C-terminal immunosuppressive domain (LCMV NPΔ340) was determined to 2.0 Å resolution. The structure indicates that LCMV NPΔ340, like the other structurally characterized arenaviral nucleoproteins, adopts the fold of an exonuclease. This structure provides a crucial three-dimensional template for functional explo...

In a study of chromosome pairing, the basic nucleoproteins of meiotic cells of Loxa flavicolis, a tropical bug, are compared with asynaptic cells of the testis and somatic cells. The normal meiotic cells are found to differ markedly from the other kinds, while only slight differences are found between asynaptic and somatic nuclei.

Isolation of nucleoproteins.-Many methods for isolating nucleoproteins involve a stage, either during the extraction or more generally in the precipitation process, which is relatively drastic and may produce an alteration in the chemical and physical properties of the nucleoprotein ; thus the majority of preparations involve precipitation of the extracted material with hydrochloric or acetic a...

The macromolecular nudeoproteins of rat liver cytoplasm give rise to a number of ultracentrifugal boundaries with sedi-mentation rates between 107 and 27 S (1). These are probably the same structures which appear as spherical particles 100 to 150 A in diameter in electron micrographs of thin sections of whole tissue (2). They contain about 45 per cent PNA (3). Since the uptake of labelled amino...