Mycoplasma agalactia was cultured in PPLO broth medium supplemented with sterilized normal horse serum in fermentor. Cultivation process continued at 37 ºC under aerobic conditions and growth was terminated after 26 hours cultured period with 2.2×109 Mycoplasma per ml. Samples were taken at different stages of growth. Culture was inactivated with 0.04 %v/v formaldehyde and the vaccine was adjuv...

Abstract Contamination of primary and cell cultures by mycoplasmas is one the main economic biological pitfalls in basic research, diagnosis manufacture biotechnological products. It a common issue which may be difficult to conduct surveillance on. Mycoplasma presence affect several physiological parameters cell, besides being considered an important source inaccurate and/or non-reproducible sc...

Almost complete (> 96%) 16S rRNA sequences from nine ruminant mycoplasmas have been determined by solid-phase DNA sequencing. Polymorphisms were found in four of the 16S rRNA sequences, which indicated the existence of two different rRNA operons. Seven polymorphisms were found in Mycoplasma agalatiae, three were found in Mycoplasma bovis, one was found in Mycoplasma alkalescens, and one was fou...

Bacterial vaginosis or non-specific vaginitis describes the disease caused by a change in the normal Flora of the vagina, which leads to the elimination of Lactobacilli, generating hydrogen peroxide and excess growth of bacteria, particularly anaerobic bacteria. This disease is the most prevalent infection of the female genital tract, and the rate of frequency of anaerobic bacteria, specificall...

One of the main problems in cell culture is mycoplasma infection. It can extensively affect cell physiology and metabolism. As the applications of cell culture increase in research, industrial production and cell therapy, more concerns about mycoplasma contamination and detection will arise. This review will provide valuable information about: 1. the ways in which cells are contaminated and the...

1. Abstract 2. Introduction 3. Classification 4. Incidence 5. Genetics 6. Mycoplasmal diseases of ruminants 6.1. Mycoplasma mycoides subsp. mycoides SC 6.2. Mycoplasma bovis 6.2.1. Pathogenesis 6.2.2. Antigenic variation 6.3. Mycoplasma dispar 7. Mycoplasmal diseases of swine 7.1. Mycoplasma hyorhinis 7.2. Mycoplasma hyopneumoniae 7.2.1. Pathogenesis 7.2.2. Adherence 7.2.3. Ciliostasis 8. Mycop...

Three hemoplasma species are recognized in domestic cats: Mycoplasma haemofelis, 'Candidatus Mycoplasma haemominutum' and 'Candidatus Mycoplasma turicensis'. We report the prevalence and hematological abnormalities of hemoplasma infection in 369 domestic cats from three different populations (blood donors, hospitalized cats and shelter cats) from Southern Brazil. Complete blood counts were perf...

background: mycoplasmas can cause acute and chronic diseases at multiple sites with wide-range complications and have been implicated as cofactors in diseases. the infections influenced form genital mycoplasmas specifically mycoplasma hominis and mycoplasma genitalium potentially affect reproductive disorders, and infertility. objective: isolation and molecular identification of mycoplasma geni...

3450 bovine from different breeds and ages were clinically examined for the presence of arthritis. 120 cases showed macroscopic evidences of arthritis. Mycoplasma spp were isolated from synovial fluid of 21 affected animals. In addition to Mycoplasma spp. Arcanobacterium pyogenes and Staphylococcus epidermidis were isolated from 2 cases. The synovial fluid was markedly increased, straw color, t...