In this study, we evaluated the susceptibility of Alphitobius diaperinus larvae and adults to saline extract (SE), lectin-rich fraction (FR), isolated lectin (MvRL) from Microgramma vacciniifolia rhizomes. To determine immediate effects, were exposed SE (10.5 mg/mL), FR (7.5 or MvRL (1.0 mg/mL) for 48 h. Live insects acetylcholinesterase (AChE) activity. The delayed effects (0.2 0.4 checked by ...

Human peripheral blood lymphocytes (PBL), obtained from patients with a variety of cancers, were incubated in vitro with phytohemagglutinin, concanavalin A, and crude or lectin-free T-cell growth factors. The lectin-activated PBL of nine patients were capable of lysing fresh autologous tumor during a 4-hr 51Cr release assay. Multiple metastases from the same patient were equivalently lysed by t...

BACKGROUND Lectins are a diverse group of carbohydrate-binding proteins exhibiting numerous biological activities and functions. METHODS Two-step serial carbohydrate affinity chromatography was used to isolate a lectin from the edible mushroom clouded agaric (Clitocybe nebularis). It was characterized biochemically, its gene and cDNA cloned and the deduced amino acid sequence analyzed. Its ac...

The effect of N-(3-phenyl-2-propenyl)-1-deoxynojirimycin (ppDNM) on the lectin binding to HIV-1 glycoprotein was analyzed by using biotinylated lectins of various sugar specificities as probes. ppDNM potentially inhibited HIV-1-induced syncytium formation and viral infectivity of HIV-1 without cytotoxicity. The lectin binding assay showed that ppDNM treatment reduced Con A binding to gp120 of H...

Background Lectins are highly important biomolecules to study several biological processes. A novel α-D-glucose/mannose specific lectin was isolated from the seeds of litchi fruits (Litchi chinensis) and its various biophysical and biochemical properties were studied. Methods Purification was done by successive Sephadex G 100 and Con A-Sepharose 4B affinity chromatography. SDS-PAGE, Surface P...

Seven Campylobacter jejuni strains were characterised by a lectin typing assay. The typing system was based on a quartz crystal microbalance technique (QCM) with four commercially available lectins (wheat germ agglutinin, Maackia amurensis lectin, Lens culinaris agglutinin, and Concanavalin A), which were chosen for their differing carbohydrate specificities. Initially, the gold surfaces of the...

Angelika Preisfeld*, Gabriele Scholten-Beck, Hans Georg Ruppel Universität Bielefeld, Fakultät für Biologie, Postfach 100131, D-33501 Bielefeld, Bundesrepublik Deutschland Z. Naturforsch. 52c, 33-41 (1997); received September 20/0ctober 29, 1996 Deficiency, Euglena gracilis, Lectin Assay, Mucilage Biochemical and ultrastructural examinations on the pellicle of autotrophically grown Eu­ glena gr...