It was reported previously that isolated human islets from individuals with type 2 diabetes mellitus (T2DM) show reduced glucose-stimulated insulin release. To assess the possibility that impaired bioenergetics may contribute to this defect, glucose-stimulated respiration (Vo(2)), glucose usage and oxidation, intracellular Ca(2+), and insulin secretion (IS) were measured in pancreatic islets is...

The pancreatic extracellular matrix (ECM) is an enormously complex construct. Previous studies underline the challenges to identify optimal combinations and ratios of individual ECM proteins for promoting survival function isolated transplanted islets. This study aimed on assessing efficiency solubilized natural extracted from juvenile pigs, unlimited donor source. Isolated human islets were cu...

The aim of the present study was to test the possible presence and expression of islet neogenesis-associated protein (INGAP) in islet cells of normal adult hamsters. Pancreata from normal male Syrian hamsters were removed to perform the following studies. (i) Western blot analysis using the cytosolic fraction from homogenates of isolated islets, exocrine tIssue and whole pancreas, and rabbit IN...

Zawalich, Walter S., Marc Bonnet-Eymard, Kathleen C. Zawalich, and Gordon C. Yaney. Chronic exposure to TPA depletes PKCa and augments Ca-dependent insulin secretion from cultured rat islets. Am. J. Physiol. 274 (Cell Physiol. 43): C1388–C1396, 1998.—The insulin secretory responses of rat islets to glucose (15 mM), 12-O-tetradecanoylphorbol 13-acetate (TPA; 500 nM), and potassium (30 mM) were d...

Creation of a bioartificial pancreas, including cell-engineered construct (CEC) formed from pancreatic islets (islets Langerhans) and biocompatible matrix mimicking the native microenvironment tissue, is one approaches to treatment type 1 diabetes mellitus (T1D). Objective: conduct preliminary in vivo studies functional efficacy intraperitoneal injection endocrine suspension rat an experimental...

BACKGROUND & OBJECTIVES Data on comparative distribution of the islet cell types in the Indian bonnet monkeys and rats are not available. The aim of the present study was to compare the arrangement of the three islet cell types in the native and in isolated rat and Indian bonnet monkey islets by immunocytochemistry. METHODS Rat islets isolated by chopped tissue collagenase digestion method an...

The insulin secretory responses of rat islets to glucose (15 mM), 12- O-tetradecanoylphorbol 13-acetate (TPA; 500 nM), and potassium (30 mM) were determined from perifused islets cultured for 22-24 h in CMRL-1066 medium (control cultured) or islets cultured in the additional presence of 500 nM TPA. Islet content of protein kinase C α (PKCα) and serine and threonine phosphoprotein patterns were ...

Successful islet isolation is crucial for islet transplantation and cell treatment for type 1 diabetes. Current isolation methods are able to obtain 500-1,000 islets per rat, which results in a waste of ≥50% of total islets. In the present study, a facile mechanical shaking method for improving islet yield (up to 1,500 per rat) was developed and summarized, which was demonstrated to be more eff...