A strongly reduced culture medium, capable of maintaining the virulence of Treponema pallidum (Nichols) for several days, was exposed to an atmosphere of 3% oxygen in nitrogen for 2-3 days before inoculation with T pallidum. By using various volumes of medium in uniform tubes a range of redox potentials (Ecal) from -94 mV to -325 mV was produced depending on the surface area-to-volume ratios of...

The anionic surfactant sodium dodecyl sulfate (SDS) was degraded by novel strain ofPseudomonas aeruginosa KGS under accession No. JQ328193, which was isolated from carwash wastewater. The purpose of this research was to study different optimization conditionsrequired for enhancing the biodegradation of sodium dodecyl sulfate P. aeruginosa KGS.Influence of different Physicochemical factors such ...

Recovery of group B streptococci (GBS) was assessed in 1,204 vaginorectal swabs stored in Amies transport medium at 4 or 21 degrees C for 1 to 4 days either by direct inoculation onto Granada agar (GA) or by culture in blood agar (BA) and GA after a selective broth enrichment (SBE) step. Following storage at 4 degrees C, GBS detection in GA was not affected after 72 h by either direct inoculati...

The optimal conditions for mycelial growth of Phellinus linteus ATCC 26710 were determined to be a log length of 20 cm, temperature of 30℃ and pH of 6.0. Mycelial growth was excellent on the mushroom complete medium, and was optimal when sucrose, mannose and glucose were supplied as carbon sources. Potassium nitrate and sodium nitrate as nitrogen sources supported good mycelial growth. To evalu...

Successful infection and replication of bacteriophages is indicative of the presence of viable bacteria. We describe here the development of a bacteriophage replication assay for the detection of Mycobacterium tuberculosis by using mycobacteriophage D29. Optimization of phage inoculate and incubation times allowed highly sensitive detection of M. bovis BCG. Fewer than 10 CFU (100 CFU/ml) were d...

medium were placed in culture tubes (16 by 150 mm). A layer of mineral oil 1 cm deep was placed on the surface of the medium, and the tubes were stoppered with cotton plugs. The tubes were then placed in an autoclave and heated for 15 min at 15 lb per in.2 During autoclaving, the culture tubes were tilted at a 200 angle to increase the exposed surface area; this reduced the problem of the mediu...

numerous date palm orchards in south kerman, iran were investigated during 2001-2003. symptoms of leaf die-back appeared as light brown stripes which began at leaf tips and extended to leaf ends. infected tissues were surface sterilized with 0.05% sodium hypochlorite and, plated on pda medium. isolated fungi were purified using hyphal tip method. on pda medium, fungal colony was fluffy, first c...

The laboratory diagnosis of tuberculosis usually relies on culture-based isolation of the causative Mycobacterium tuberculosis bacteria. We developed and evaluated the performance of MOD9, a new blood-free derivative of the MOD4 solid medium on which we previously reported for the isolation and culture of mycobacteria. First, inoculation of Lowenstein-Jensen medium with 21 M. tuberculosis isola...

background diazinon is one of the most widely used organophosphrous pesticides in the world may affect animals and human. objectives this study was done to assess whether pseudomonas plecoglossicida can grow on diazinon as the sole carbon metabolic source in laboratory and agricultural soil. materials and methods serial dilutions of pure and commercial diazinon in minimal salt medium were made ...

Ninety-nine clinical staphylococcal isolates (58 coagulase-negative Staphylococcus spp. [CoNS] and 41 Staphylococcus aureus isolates) were evaluated for susceptibility to oxacillin. The following susceptibility testing methods, media, and incubation conditions were studied: agar dilution by using Mueller-Hinton (MH) medium (Difco) supplemented with either 0, 2, or 4% NaCl and incubation at 30 o...